Frölich K, Meyer H H, Pielowski Z, Ronsholt L, von Seck-Lanzendorf S, Stolte M
Institute for Zoo Biology and Wildlife Research Berlin, Germany.
J Wildl Dis. 1996 Apr;32(2):280-5. doi: 10.7589/0090-3558-32.2.280.
A study of European brown hare syndrome (EBHS) was conducted in Poland (Czempin). From April 1993 until February 1994, 100 blood and 78 spleen samples of European brown hares (Lepus europaeus) were tested for prevalence of EBHS and rabbit hemorrhagic disease (RHDV) antibodies and EBHS virus antigen with two enzyme-linked immunosorbent assay (ELISA) test kits. Thirty-eight of 100 serum samples were positive for EBHS, and six (7.6%) of 78 of the spleen materials were-antigen positive for EBHS virus. Three (3%) of the sera were positive against RHDV, whereas two of these also were seropositive for EBHSV. European brown hare syndrome virus seropositive hares were most frequently found from April to September. Based on negative staining electron microscopy investigations of liver and spleen homogenates of all antigen-positive hares, we observed caliciviruses in only one animal. For histopathological investigations organ specimens were available from 98 hares. Histopathological findings corresponded with the clinical picture of chronic EBHS. A pathohistological picture consistent with EBHS was found in 22 (22%) of investigated hares and corresponded in 50% of the animals which reacted positively in the EBHSV antigen-ELISA and in 29% of the animals which reacted positively in the EBHSV antibody-ELISA. These results from western Poland are the first that caliciviruses are present in European brown hare population in Eastern Europe and may be one of the causes for increased mortality in the Polish hare population over the past 10 years.
在波兰（琴平）开展了一项关于欧洲棕兔综合征（EBHS）的研究。从1993年4月至1994年2月，采用两种酶联免疫吸附测定（ELISA）试剂盒，对100份欧洲棕兔（欧洲野兔）的血液样本和78份脾脏样本进行了检测，以确定欧洲棕兔综合征（EBHS）和兔出血性疾病（RHDV）抗体以及EBHS病毒抗原的流行情况。100份血清样本中有38份EBHS呈阳性，78份脾脏样本中有6份（7.6%）EBHS病毒抗原呈阳性。3份（3%）血清对RHDV呈阳性，其中2份对EBHSV也呈血清阳性。欧洲棕兔综合征病毒血清阳性的野兔最常见于4月至9月。基于对所有抗原阳性野兔的肝脏和脾脏匀浆进行的负染色电子显微镜检查，我们仅在一只动物中观察到杯状病毒。有98只野兔的器官标本可用于组织病理学研究。组织病理学发现与慢性EBHS的临床表现相符。在22只（22%）被调查的野兔中发现了与EBHS一致的病理组织学图像，在EBHSV抗原ELISA检测呈阳性的动物中有50%以及在EBHSV抗体ELISA检测呈阳性的动物中有29%与之相符。来自波兰西部的这些结果首次表明，东欧欧洲棕兔种群中存在杯状病毒，这可能是过去10年波兰野兔种群死亡率上升的原因之一。
