Frölich Kai, Kujawski Olgierd E J Graf, Rudolph Michael, Ronsholt Leif, Speck Stephanie
Institute for Zoo and Wildlife Research Berlin, Alfred-Kowalke-Str. 17, P.O. Box 601103, 10252 Berlin, Germany.
J Wildl Dis. 2003 Jan;39(1):121-4. doi: 10.7589/0090-3558-39.1.121.
From 1998 to 2000, serum samples of 80 shot European brown hares (Lepus europaeus) from Argentina were examined for antibodies against European brown hare syndrome virus (EBHSV) and 80 spleen samples were tested for EBHSV-antigen by enzyme linked immunosorbent assay (ELISA). Nine hares were positive for EBHSV-antigen. Antibodies against EBHSV were detected in only one individual. Based on negative staining electron microscopy of spleen homogenates, we observed calicivirus in one of five EBHSV-antigen positive hares. However, EBHS has not been reported to cause abnormal mortality in these hares. This is the first report of antibodies to EBHSV, EBHSV-antigen, and electron microscopy findings in free-ranging European brown hares from South America.
1998年至2000年期间，对来自阿根廷的80只射杀的欧洲棕兔（欧洲野兔）的血清样本进行了检测，以检测其针对欧洲棕兔综合征病毒（EBHSV）的抗体，并通过酶联免疫吸附测定（ELISA）对80份脾脏样本进行了EBHSV抗原检测。9只野兔的EBHSV抗原呈阳性。仅在一只个体中检测到针对EBHSV的抗体。基于脾脏匀浆的负染色电子显微镜观察，我们在五只EBHSV抗原阳性野兔中的一只中观察到杯状病毒。然而，尚未有报道称EBHS会导致这些野兔出现异常死亡。这是关于南美野生欧洲棕兔中EBHSV抗体、EBHSV抗原及电子显微镜检查结果的首次报告。
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