Frölich Kai, Fickel Jörns, Ludwig Arne, Lieckfeldt Dietmar, Streich Wolf Jürgen, Jurcik Rastislav, Slamecka Jaroslav, Wibbelt Gudrun
Leibniz-Institute for Zoo and Wildlife Research, PO Box 601103, 10252 Berlin, Germany.
J Wildl Dis. 2007 Jan;43(1):89-96. doi: 10.7589/0090-3558-43.1.89.
Investigations regarding European brown hare syndrome virus (EBHSV) in European brown hares (Lepus europaeus) in Slovakia were undertaken in order to detect the possible presence of EBHSV and to evaluate its phylogenetic position. Liver and/or serum samples were obtained from 135 European brown hares shot by hunters in eight regional hunting areas. From 36 animals corresponding liver and serum samples were available; from the remaining 49 and 50 animals only liver or serum samples were examined, respectively. Samples were tested for antibodies against EBHSV and for viral RNA by reverse transcriptase-polymerase chain reaction (RT-PCR) and RT-PCR products were subsequently sequenced. Additionally, matrilinear hare haplotypes were analyzed in order to detect potential familial susceptibility to EBHSV. Sixty-three of 86 sera contained antibodies against EBHSV, whereas 15 of 85 liver samples were PCR positive. Of the latter, 14 were sequenced, revealing three new strains of EBHSV. Fifteen different matrilinear haplotypes were identified, but no correlation was found between haplotype and susceptibility to EBHSV infection. Our findings confirmed the existence of EBHSV in Slovakia and reinforce the need for determining EBHSV status when importing hares for restocking.
为了检测欧洲棕色野兔综合征病毒（EBHSV）在斯洛伐克欧洲棕色野兔（Lepus europaeus）中可能的存在情况并评估其系统发育位置，开展了相关调查。从八个区域狩猎区被猎人射杀的135只欧洲棕色野兔身上获取了肝脏和/或血清样本。在36只动物身上同时获取了相应的肝脏和血清样本；对于其余49只和50只动物，分别仅检测了肝脏或血清样本。通过逆转录聚合酶链反应（RT-PCR）检测样本中针对EBHSV的抗体和病毒RNA，并对RT-PCR产物进行测序。此外，分析了母系野兔单倍型，以检测对EBHSV的潜在家族易感性。86份血清中有63份含有针对EBHSV的抗体，而85份肝脏样本中有15份PCR呈阳性。对后者中的14份进行了测序，发现了三种EBHSV新毒株。鉴定出15种不同的母系单倍型，但未发现单倍型与EBHSV感染易感性之间存在相关性。我们的研究结果证实了EBHSV在斯洛伐克的存在，并强化了在进口野兔用于补充种群时确定EBHSV状况的必要性。
