Chakrabarty A M
J Bacteriol. 1972 Nov;112(2):815-23. doi: 10.1128/jb.112.2.815-823.1972.
The genetic basis of the biodegradation of salicylate in Pseudomonas putida R1 has been studied. This strain utilizes the meta pathway for oxidizing salicylate through formation of catechol and 2-hydroxymuconic semialdehyde. The enzymes of the meta pathway are induced by salicylate but not by catechol, and the genes specifying these enzymes are clustered. The gene cluster can be eliminated from some salicylate-positive cells by treatment with mitomycin C and appears to exist inside the cell as an extrachromosomal element. This extrachromosomal gene cluster, termed the SAL plasmid, can be transferred by conjugation from P. putida R1 to a variety of other Pseudomonas species.
对恶臭假单胞菌R1中水杨酸盐生物降解的遗传基础进行了研究。该菌株利用间位途径通过形成儿茶酚和2-羟基粘康酸半醛来氧化水杨酸盐。间位途径的酶由水杨酸盐诱导而非儿茶酚诱导,并且指定这些酶的基因成簇存在。通过丝裂霉素C处理,可以从一些水杨酸盐阳性细胞中消除该基因簇,并且该基因簇似乎作为一种染色体外元件存在于细胞内。这种染色体外基因簇称为SAL质粒,可以通过接合作用从恶臭假单胞菌R1转移到多种其他假单胞菌属物种中。