对添加蔗糖或半胱氨酸的血培养基的评估。
Evaluation of blood culture media supplemented with sucrose or with cysteine.
作者信息
Washington J A, Hall M M, Warren E
出版信息
J Clin Microbiol. 1975 Jan;1(1):79-81. doi: 10.1128/jcm.1.1.79-81.1975.
Abstract
A total of 5,883 blood samples from patients with suspected bacteremia were inoculated concurrently into each of three media under vacuum with CO2: tryptic soy broth (TSB) with sodium polyanetholesulfonate (SPS), TSB with SPS and cysteine, and TSB with SPS and sucrose. There were 395 positive cultures, excluding presumed contaminants. No significant differences were noted with the addition of cysteine to TSB with SPS, and no streptococcal mutants requiring thiol groups were isolated. Haemophilus, Staphylococcus aureus, and bacteriodaceae were isolated more frequently (P less than 0.05) in the absence of sucrose. The addition of sucrose to TSB containing SPS did not significantly increase the rate of positivity or the time interval to detection of positivity of any group of bacteria.
摘要
总共5883份疑似菌血症患者的血样同时接种到三种含二氧化碳的真空培养基中：含多粘菌素B磺酸钠（SPS）的胰蛋白胨大豆肉汤（TSB）、含SPS和半胱氨酸的TSB以及含SPS和蔗糖的TSB。排除假定的污染物后，有395份培养物呈阳性。在含SPS的TSB中添加半胱氨酸未发现显著差异，也未分离出需要巯基的链球菌突变体。在没有蔗糖的情况下，嗜血杆菌、金黄色葡萄球菌和拟杆菌科的分离频率更高（P小于0.05）。在含SPS的TSB中添加蔗糖并未显著提高任何一组细菌的阳性率或检测到阳性的时间间隔。
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