Boutonnier A, Nato F, Bouvet A, Lebrun L, Audurier A, Mazie J C, Fournier J M
Institut Pasteur, Paris, France.
J Clin Microbiol. 1989 May;27(5):989-93. doi: 10.1128/jcm.27.5.989-993.1989.
Monoclonal antibodies (MAbs) reactive with serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus have been used to test, by enzyme-linked immunosorbent assay (ELISA), blood culture fluids for the presence of S. aureus. A total of 748 blood cultures from 665 patients yielding 706 bacterial isolates belonging to more than 26 bacterial species were studied. All blood cultures containing bacterial strains belonging to species other than S. aureus were negative in ELISA. All 23 blood cultures containing serotype 5 S. aureus were positive in ELISA with the corresponding MAb. Out of 20 blood cultures containing serotype 8 S. aureus, 19 were positive with the corresponding MAb. All 5 blood cultures containing nontypeable S. aureus were negative in ELISA with both MAbs. This method provides reliable identification of serotype 5 or serotype 8 S. aureus by direct testing of blood culture fluids with ELISA.
已使用与金黄色葡萄球菌5型和8型荚膜多糖反应的单克隆抗体（MAb），通过酶联免疫吸附测定（ELISA）检测血培养液中是否存在金黄色葡萄球菌。对665名患者的748份血培养物进行了研究，共获得706株属于26种以上细菌的细菌分离株。所有含有非金黄色葡萄球菌菌株的血培养物在ELISA中均为阴性。所有23份含有5型金黄色葡萄球菌的血培养物在ELISA中与相应的单克隆抗体反应均呈阳性。在20份含有8型金黄色葡萄球菌的血培养物中，19份与相应的单克隆抗体反应呈阳性。所有5份含有不可分型金黄色葡萄球菌的血培养物在ELISA中与两种单克隆抗体反应均为阴性。该方法通过用ELISA直接检测血培养液，可对5型或8型金黄色葡萄球菌进行可靠鉴定。
