Murray P R
J Clin Microbiol. 1985 Apr;21(4):481-5. doi: 10.1128/jcm.21.4.481-485.1985.
The value of incubating blood culture broths for more than 7 days was analyzed. A total of 20,155 blood cultures, consisting of a vented Roche tryptic soy broth (R-TSB) bottle and an unvented Difco thiol broth bottle, was processed; 2,509 organisms were recovered in the R-TSB bottles, and 1,865 organisms in the thiol bottles. Only 32 organisms isolated in the R-TSB bottles and 10 organisms isolated in the thiol bottles were detected after incubation for more than 7 days; 15 of the 32 isolates in the R-TSB bottles and all 10 of the isolates in the thiol bottles were either recovered in other blood cultures or were not considered to be clinically significant. Thus, incubation of the R-TSB bottles and unvented thiol bottles for more than 7 days is unnecessary for the detection of most clinically significant septicemias.
分析了血培养肉汤培养7天以上的价值。共处理了20155份血培养样本，包括一个通气的罗氏胰蛋白胨大豆肉汤（R-TSB）瓶和一个不通气的迪夫科硫醇肉汤瓶；在R-TSB瓶中分离出2509株微生物，在硫醇瓶中分离出1865株微生物。在培养7天以上后，R-TSB瓶中仅检测到32株分离菌，硫醇瓶中仅检测到10株分离菌；R-TSB瓶中的32株分离菌中有15株以及硫醇瓶中的所有10株分离菌在其他血培养中被检出或被认为无临床意义。因此，对于大多数有临床意义的败血症检测而言，将R-TSB瓶和不通气的硫醇瓶培养7天以上并无必要。
