多酚对体外培养的大鼠股骨组织钙含量和碱性磷酸酶活性的影响。

Effect of polyphenols on calcium content and alkaline phosphatase activity in rat femoral tissues in vitro.

作者信息

Yamaguchi M, Jie Z

机构信息

Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.

出版信息

Biol Pharm Bull. 2001 Dec;24(12):1437-9. doi: 10.1248/bpb.24.1437.

DOI:10.1248/bpb.24.1437

PMID:11767119

Abstract

The effect of various polyphenols on calcium content and alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues of young rats in vitro was investigated. Bone tissues were cultured for 24 h in serum-free Dulbecco's modified Eagle's medium containing either vehicle or various polyphenols (10(-7) - 10(-4) M). The presence of genistein (10(-6) - 10(-4) M) caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues. Resveratrol (10(-4) m) decreased metaphyseal calcium content significantly, and it (10(-6) - 10(-4) M) had a significant inhibitory effect on diaphyseal enzyme activity. Epigallocatechin gallate (EGCg; 10(-4) M) significantly inhibited alkaline phosphatase activity in the diaphyseal and metaphyseal tissues. EGCg (10(-7) - 10(-4) M) had no effect on bone calcium content. Meanwhile, glycitein, quercetin, or catechin in the range of 10(-7) to 10(-4) ml did not have an effect on calcium content and alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues. The present study suggests that a phytoestrogen genistein has a unique anabolic effect on bone calcification in vitro.

摘要

研究了多种多酚对幼鼠股骨干和干骺端组织中钙含量及碱性磷酸酶活性的体外影响。将骨组织在含有载体或多种多酚（10⁻⁷ - 10⁻⁴ M）的无血清杜尔贝科改良伊格尔培养基中培养24小时。染料木黄酮（10⁻⁶ - 10⁻⁴ M）的存在导致股骨干和干骺端组织中的钙含量及碱性磷酸酶活性显著增加。白藜芦醇（10⁻⁴ m）显著降低了干骺端钙含量，并且它（10⁻⁶ - 10⁻⁴ M）对骨干酶活性有显著抑制作用。表没食子儿茶素没食子酸酯（EGCg；10⁻⁴ M）显著抑制了骨干和干骺端组织中的碱性磷酸酶活性。EGCg（10⁻⁷ - 10⁻⁴ M）对骨钙含量无影响。同时，10⁻⁷至10⁻⁴ ml范围内的大豆黄素、槲皮素或儿茶素对股骨干和干骺端组织中的钙含量及碱性磷酸酶活性无影响。本研究表明，植物雌激素染料木黄酮在体外对骨钙化具有独特的合成代谢作用。