Yamaguchi M, Gao Y H
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka City, Japan.
Res Exp Med (Berl). 1997;197(2):101-7. doi: 10.1007/s004330050059.
Whether the anabolic effect of genistein on bone metabolism in the femoral-metaphyseal tissues of elderly rats is inhibited by the anti-estrogen tamoxifen was investigated in vitro. Bone tissues were cultured for 24 h in the medium containing either vehicle or genistein. The presence of genistein (10(-6) and 10(-5) M) caused a significant increase in calcium content, alkaline phosphatase activity and DNA content in the bone tissues. The effect of genistein (10(-5) M) in increasing bone alkaline phosphatase activity and DNA content was equal to the stimulatory effect of 17 beta-estradiol (10(-10) and 10(-9) M) on the bone components. The genistein effect was not significantly enhanced by the presence of 17 beta-estradiol (10(-10) and 10(-9) M). The anti-estrogen tamoxifen (10(-7) M) completely blocked the effect of 17 beta-estrogen in increasing bone alkaline phosphatase activity and DNA content. The genistein (10(-5) M)-induced increases in bone alkaline phosphatase activity and DNA content were completely abolished by the presence of tamoxifen (10(-7) and 10(-6) M), although tamoxifen itself had no effect on the bone components. The present study demonstrated that the anabolic effect of genistein on bone components is inhibited by anti-estrogen tamoxifen. The effect of genistein may be mediated through estrogen-like action.
在体外研究了抗雌激素他莫昔芬是否会抑制金雀异黄素对老年大鼠股骨-干骺端组织骨代谢的合成代谢作用。将骨组织在含有赋形剂或金雀异黄素的培养基中培养24小时。金雀异黄素（10^(-6)和10^(-5) M）的存在导致骨组织中钙含量、碱性磷酸酶活性和DNA含量显著增加。金雀异黄素（10^(-5) M）增加骨碱性磷酸酶活性和DNA含量的作用与17β-雌二醇（10^(-10)和10^(-9) M）对骨成分的刺激作用相当。17β-雌二醇（10^(-10)和10^(-9) M）的存在并未显著增强金雀异黄素的作用。抗雌激素他莫昔芬（10^(-7) M）完全阻断了17β-雌激素增加骨碱性磷酸酶活性和DNA含量的作用。他莫昔芬（10^(-7)和10^(-6) M）的存在完全消除了金雀异黄素（10^(-5) M）诱导的骨碱性磷酸酶活性和DNA含量的增加，尽管他莫昔芬本身对骨成分没有影响。本研究表明，抗雌激素他莫昔芬会抑制金雀异黄素对骨成分的合成代谢作用。金雀异黄素的作用可能是通过类似雌激素的作用介导的。
