Penny J E, Kukums J R, Eadie M J, Tyrer J H
J Neurol Sci. 1975 Oct;26(2):179-85. doi: 10.1016/0022-510x(75)90030-1.
Cytophotometric measurements of the activities of 5 oxidative enzymes (succinate, malate, lactate, NAD+-linked isocitrate and NADH dehydrogensases) have been made in anterior horn cells of the lumbar and cervical spinal cord of the rabbit. The thickness of tissue sections was measured by an interference microscope and various optical and chemical precautions were taken to diminish the possible errors that might be involved in cytophotometry. The findings of the study indicated a unimodal distribution of the activities of all of the enzymes studied in anterior horn cells, though there was a wide range of enzyme concentration among different cells. Thus the findings in the rabbit are consistent with the "constant proportion" hypothesis of the activity of certain oxidative enzymes, and are contrary to a previous finding that there may be two populations of succinate dehydrogenase-containing anterior horn cell.
已对兔腰段和颈段脊髓前角细胞中5种氧化酶（琥珀酸脱氢酶、苹果酸脱氢酶、乳酸脱氢酶、NAD⁺连接的异柠檬酸脱氢酶和NADH脱氢酶）的活性进行了细胞光度测量。用干涉显微镜测量组织切片的厚度，并采取了各种光学和化学预防措施，以减少细胞光度测量中可能涉及的误差。该研究结果表明，在所研究的前角细胞中，所有酶的活性均呈单峰分布，尽管不同细胞之间的酶浓度范围很广。因此，兔的研究结果与某些氧化酶活性的“恒定比例”假说一致，与之前关于可能存在两类含琥珀酸脱氢酶的前角细胞的发现相反。
