Moore J S, Zaki M H
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, University of Pennsylvania Cancer Center, USA.
Clin Lab Med. 2001 Dec;21(4):795-809.
Cytokines play an important role in human health and disease. Monitoring their effects and detecting alterations in the complex balance of cytokines within a patient will undoubtedly become increasingly common in the clinical laboratory. Because of the complexity of the network interactions, multiple assays measuring soluble cytokines ("what"), cytokine-producing cells ("who"), surface receptors ("where"), and function (how) simultaneously are necessary to provide clinically useful information. The explosion of reagents and applications for use in the clinical flow cytometry laboratory makes this the perfect setting to perform the multidimensional studies required. Clinical cytokine network cytometry exemplifies the power of multiparameter, high throughput technologies that will change the face of clinical laboratories in the twenty-first century.
细胞因子在人类健康和疾病中发挥着重要作用。监测它们的作用并检测患者体内细胞因子复杂平衡的变化，无疑将在临床实验室中变得越来越普遍。由于网络相互作用的复杂性，需要多种检测方法同时测量可溶性细胞因子（“是什么”）、产生细胞因子的细胞（“是谁”）、表面受体（“在哪里”）和功能（“如何”），以提供具有临床意义的信息。临床流式细胞术实验室中试剂和应用的激增，使其成为进行所需多维研究的理想场所。临床细胞因子网络流式细胞术体现了多参数、高通量技术的强大力量，这些技术将改变21世纪临床实验室的面貌。
