King J A, Snow M, Smail D A, Raynard R S
FRS Marine Laboratory, Aberdeen, Scotland, UK.
Dis Aquat Organ. 2001 Nov 8;47(2):81-6. doi: 10.3354/dao047081.
A surveillance programme was initiated on the occurrence and distribution of viral haemorrhagic septicaemia virus (VHSV) in wild marine fish. Six research cruises were undertaken in an 18 mo period during 1997 and 1998, covering the North Sea, the Atlantic waters off the north and west coasts of Scotland and the Irish Sea. A total of 19,293 fish were sampled from 23 different species including cod, haddock, Norway pout, herring and sprat. Individual fish lengths were recorded and the fish were checked for lesions, haemorrhaging and other signs of disease. Pools of organ samples were taken for virus assay. The majority of fish sampled did not display clinical signs indicative of viral haemorrhagic septicaemia. A small number of cod were found with skin lesions and haddock with skin haemorrhaging. Of the 2081 organ and skin sample pools collected, 21 tested positive for VHSV by tissue culture and enzyme-linked immunosorbent assay. Seventeen of the isolates originated from Norway pout Trisopterus esmarkii, one from cod Gadus morhua (skin lesion), one from herring Clupea harengus, one from whiting Merlangius merlangus, and one from a previously unreported host species, poor cod Trisopterus minutus.
针对野生海洋鱼类中病毒性出血性败血症病毒（VHSV）的发生和分布情况启动了一项监测计划。在1997年和1998年的18个月期间进行了6次研究巡航，覆盖北海、苏格兰北部和西部海岸外的大西洋水域以及爱尔兰海。共从包括鳕鱼、黑线鳕、挪威长鳍鳕、鲱鱼和黍鲱在内的23个不同物种中采集了19293条鱼。记录了每条鱼的长度，并检查鱼是否有损伤、出血和其他疾病迹象。采集器官样本池进行病毒检测。大多数采样的鱼没有表现出病毒性出血性败血症的临床症状。发现少数鳕鱼有皮肤损伤，黑线鳕有皮肤出血。在收集的2081个器官和皮肤样本池中，有21个通过组织培养和酶联免疫吸附测定法检测出VHSV呈阳性。其中17个分离株来自挪威长鳍鳕Trisopterus esmarkii，1个来自鳕鱼Gadus morhua（皮肤损伤），1个来自鲱鱼Clupea harengus，1个来自牙鳕Merlangius merlangus，还有1个来自一个先前未报告的宿主物种——小鳍鳕Trisopterus minutus。
