Liu T, Yang P, Wang C, Zhang N, Zhang T
Department of Otolaryngology, First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China.
Chin Med J (Engl). 2000 Oct;113(10):938-41.
Superantigens are potent inflammatory stimuli which derive from pathogenic microbes such as bacteria, viruses and protozoa. The aim of this study was to investigate the role of superantigens on the function of rabbit maxillary sinus epithelium.
Twenty New Zealand white rabbits were divided into 4 groups. Rabbit sinus mucosa was separated under a surgical microscope and mounted in Ussing chambers to record short circuit current, conductance and permeability to horseradish peroxidase (HRP). Group A was used as normal control. Group B was stimulated with an injection of superantigen into the sinus for 4 hours. The sinus mucosa of Group C was stimulated by the addition of tumor necrosis factor alpha (TNF-alpha) into Ussing chambers. Group D sinus mucosa was stimulated by superantigen after pretreatment with anti-TNF-alpha antibody.
Superantigen evoked increases in sinus epithelial cell baseline short circuit current, conductance and permeability to HRP stimulated by the addition of TNF-alpha into Ussing chambers. These were similar to results from superantigen stimulation in vivo. The effect of superantigen on sinus epithelial cells could be blocked by pretreatment with anti-TNF-alpha antibody.
Superantigen affected the function of sinus epithelial cells, including the capability of epithelial defensive barrier, which might be mediated by TNF-alpha.
超抗原是源自细菌、病毒和原生动物等致病微生物的强效炎症刺激物。本研究旨在探讨超抗原对兔上颌窦上皮功能的作用。
将20只新西兰白兔分为4组。在手术显微镜下分离兔鼻窦黏膜，并将其安装在尤斯灌流小室中，以记录短路电流、电导和对辣根过氧化物酶（HRP）的通透性。A组作为正常对照。B组通过向鼻窦内注射超抗原刺激4小时。C组鼻窦黏膜通过在尤斯灌流小室中添加肿瘤坏死因子α（TNF-α）进行刺激。D组鼻窦黏膜在预先用抗TNF-α抗体处理后用超抗原刺激。
超抗原使鼻窦上皮细胞的基线短路电流、电导以及在尤斯灌流小室中添加TNF-α刺激后对HRP的通透性增加。这些结果与体内超抗原刺激的结果相似。超抗原对鼻窦上皮细胞的作用可通过预先用抗TNF-α抗体处理来阻断。
超抗原影响鼻窦上皮细胞的功能，包括上皮防御屏障功能，这可能由TNF-α介导。
