Yang P C, Liu T, Wang C S, Zhang N Z, Tao Z D
Department of Otolaryngology, First Affiliated Hospital, Shanxi Medical University, Taiyuan and Yale-Xiang Hospital, Hunan Medical University, Changsha.
Otolaryngol Head Neck Surg. 2000 Jul;123(1 Pt 1):120-3. doi: 10.1067/mhn.2000.102808.
The aim of this study was to investigate the impact of staphylococcal enterotoxin B (SEB), a model superantigen, on the physiologic functions of rabbit maxillary sinus epithelium. Rabbit sinus mucosae were separated under a surgical microscope and mounted in Ussing chambers to record short-circuit current, conductance, and permeability to horseradish peroxidase. The results showed that SEB evoked increases in sinus epithelial cell baseline short-circuit current, conductance, and permeability to horseradish peroxidase. When tumor necrosis factor-alpha (TNF-alpha) was added to the Ussing chambers, we got results similar to those obtained by SEB stimulation in vitro; the effects of SEB on sinus epithelial cells could be blocked by pretreatment with anti-TNF-alpha antibody. These results demonstrate that SEB is able to alter the function of sinus epithelial cells and to affect the capability of the epithelial defensive barrier, which may be mediated by TNF-alpha.
本研究的目的是探讨作为典型超抗原的葡萄球菌肠毒素B（SEB）对兔上颌窦上皮生理功能的影响。在手术显微镜下分离兔鼻窦黏膜，并将其安装在尤斯灌流小室中，以记录短路电流、电导和对辣根过氧化物酶的通透性。结果显示，SEB引起鼻窦上皮细胞基线短路电流、电导以及对辣根过氧化物酶通透性增加。当向尤斯灌流小室中加入肿瘤坏死因子-α（TNF-α）时，我们得到了与体外SEB刺激相似的结果；用抗TNF-α抗体预处理可阻断SEB对鼻窦上皮细胞的作用。这些结果表明，SEB能够改变鼻窦上皮细胞的功能并影响上皮防御屏障的能力，这可能由TNF-α介导。
