Xu L, Yang X, Xie L
Key Laboratory of Carcinogenesis of Chineses Ministry of Public Health, Hunan Medical University, Changsha, 410078, China.
Zhonghua Zhong Liu Za Zhi. 2000 Jan;22(1):17-8.
To develop a sensitive non-isotopic method for in situ detection of Epstein-Barr virus (EBV) in paraffin-embedded section of nasopharyngeal carcinoma (NPC) tissue.
The EBV BamHI-W fragment labeled with digoxigenin as probe was used to perform in situ hybridization and PCR in situ hybridization (in situ PCR).
The method of in situ PCR for the detection of EBV in NPC tissue was proved to be much more sensitive than in situ hybridization.
Hybridization in situ of PCR product is useful to detect EBV in NPC tissues.
建立一种灵敏的非同位素方法，用于原位检测鼻咽癌（NPC）组织石蜡包埋切片中的爱泼斯坦-巴尔病毒（EBV）。
以地高辛标记的EBV BamHI-W片段为探针，进行原位杂交和聚合酶链反应原位杂交（原位PCR）。
证明原位PCR检测NPC组织中EBV的方法比原位杂交灵敏得多。
PCR产物原位杂交有助于检测NPC组织中的EBV。
