Hwang T Z, Jin Y T, Tsai S T
Department of Otolaryngology, National Cheng Kung University Medical College, Tainan, Taiwan.
Anticancer Res. 1998 Nov-Dec;18(6B):4581-4.
Epstein-Barr virus (EBV) has a well-established association with nasopharyngeal carcinoma (NPC), while its association with sinonasal carcinoma is still controversial. The role of EBV in sinonasal carcinoma and the potential value of EBER is situ hybridization in differentiating carcinomas originating from these anatomically neighboring regions were investigated. EBER in situ hybridization was applied to detect EBV in the specimens of surgically proved sinonasal carcinoma and the biopsies of carcinoma of the nasopharynx. EBER in situ hybridization was performed on paraffin embedded tissues by using PCR-derived, digoxigenin-labeled EBER-1 DNA probes. EBV was detected in 2 of 31 (6.5%) surgical specimens of sinonasal carcinoma, including 1 keratinizing squamous cell carcinoma and 1 adenocarcinoma. EBV was detected in all of 31 (100%) biopsies of carcinomas of the nasopharynx, including 1 keratining squamous cell carcinoma, 15 nonkeratinizing carcinomas, 14 undifferentiated carcinomas, and 1 adenocarcinoma. The present study does not support the role of EBV in the development of sinonasal carcinoma as does in carcinoma of the nasopharynx. EBER in situ hybridization is a useful adjunct for differentiating carcinomas originating from the sinonasal region and the nasopharynx.
爱泼斯坦-巴尔病毒（EBV）与鼻咽癌（NPC）之间的关联已得到充分证实，而其与鼻窦癌的关联仍存在争议。本研究探讨了EBV在鼻窦癌中的作用以及EBER原位杂交在鉴别源自这些解剖学相邻区域的癌症中的潜在价值。应用EBER原位杂交技术检测手术证实的鼻窦癌标本及鼻咽癌活检组织中的EBV。采用PCR衍生的地高辛标记EBER-1 DNA探针，对石蜡包埋组织进行EBER原位杂交。在31例鼻窦癌手术标本中，2例（6.5%）检测到EBV，其中1例为角化性鳞状细胞癌，1例为腺癌。在31例鼻咽癌活检组织中均检测到EBV（100%），包括1例角化性鳞状细胞癌、15例非角化性癌、14例未分化癌和1例腺癌。本研究不支持EBV在鼻窦癌发生中发挥如在鼻咽癌中那样的作用。EBER原位杂交是鉴别源自鼻窦区域和鼻咽部癌症的有用辅助手段。
