马巴贝斯虫培养促生长因子的分析

Analysis of a growth-promoting factor for Babesia caballi cultivation.

作者信息

Ikadai H, Martin M D, Nagasawa H, Fujisaki K, Suzuki N, Mikami T, Kudo N, Oyamada T, Igarashi I

机构信息

Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.

出版信息

J Parasitol. 2001 Dec;87(6):1484-6. doi: 10.1645/0022-3395(2001)087[1484:AOAGPF]2.0.CO;2.

DOI:10.1645/0022-3395(2001)087[1484:AOAGPF]2.0.CO;2

PMID:11780845

Abstract

Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO2 in air. Growth of B. caballi was dependent of the suitable concentration (10-20%) of GF21. Therefore, GF21 was suggested as the growth-promoting factor for B. caballi. However, GIT medium did not support the growth of parasites from cryopreserved stabilates, and serum supplementation was essential for the retrieval of parasites.

摘要

对无血清培养基进行了检测，以培养马巴贝斯虫。补充了Daigo's GF21（GF21）的Daigo's T（DT）基础培养基或已含有GF21的GIT培养基，能在37℃、含5%二氧化碳的潮湿空气中支持寄生虫繁殖。马巴贝斯虫的生长依赖于GF21的合适浓度（10 - 20%）。因此，GF21被认为是马巴贝斯虫的生长促进因子。然而，GIT培养基不支持来自冷冻保存虫株的寄生虫生长，血清补充对于寄生虫复苏至关重要。