Ikadai Hiromi, Tanaka Tetsuya, Shibahara Nona, Tanaka Hiroko, Matsuu Aya, Kudo Noboru, Shimazaki Kei-ichi, Igarashi Ikuo, Oyamada Takashi
Department of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Am J Trop Med Hyg. 2005 Oct;73(4):710-2.
Lactoferrin (LF) is an important biologic molecule with many functions, one of which is antimicrobial defense. We evaluated the growth-inhibiting effects of four types of LF (native LF, Fe(+3)-bound [holo] LF, Fe(+3)-free [apo] LF, and LF hydrolyzate) on the in vitro growth of Babesia caballi and B. equi. The growth of B. caballi was significantly suppressed in media containing apo LF, but was not inhibited in media containing native LF, holo LF, or LF hydrolyzate. The growth of B. equi was not inhibited by media containing native LF, holo LF, or apo LF. These data indicate that apo LF had the strongest inhibitory effect on B. caballi. This may have been caused by inactivation or inhibition of a growth factor in the culture medium.
乳铁蛋白（LF）是一种具有多种功能的重要生物分子，其中之一是抗菌防御。我们评估了四种类型的乳铁蛋白（天然乳铁蛋白、铁（+3）结合型[全铁]乳铁蛋白、无铁（+3）型[脱铁]乳铁蛋白和乳铁蛋白水解产物）对马巴贝斯虫和驽巴贝斯虫体外生长的抑制作用。在含有脱铁乳铁蛋白的培养基中，马巴贝斯虫的生长受到显著抑制，但在含有天然乳铁蛋白、全铁乳铁蛋白或乳铁蛋白水解产物的培养基中未受到抑制。含有天然乳铁蛋白、全铁乳铁蛋白或脱铁乳铁蛋白的培养基对驽巴贝斯虫的生长没有抑制作用。这些数据表明，脱铁乳铁蛋白对马巴贝斯虫具有最强的抑制作用。这可能是由于培养基中生长因子的失活或抑制所致。
