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Isolation and characterization of cDNA complementary to transient maternal poly(A)+ RNA from the Drosophila oocyte.

作者信息

Goldstein E S, Arthur C G

出版信息

Biochim Biophys Acta. 1979 Dec 17;565(2):265-74. doi: 10.1016/0005-2787(79)90204-1.

DOI:10.1016/0005-2787(79)90204-1
PMID:117835
Abstract

cDNA complementary to total oocyte poly(A)+ RNA from Drosophila melanogaster was enriched for sequences complementary to transient maternal sequences; that is, those sequences which disappear from the oocyte during subsequent. A seven- to ten-fold enrichment factor was obtained, from 5.3% to about 50% of the total cDNA. Kinetic analysis of this enriched fraction indicates that the transient maternal sequences include 44 +/- 14 different sequences.

摘要

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