Biessmann H
Nucleic Acids Res. 1980 Dec 20;8(24):6099-111. doi: 10.1093/nar/8.24.6099.
Steady state concentrations of individual RNA sequences in poly(A) nuclear and cytoplasmic RNA populations of Drosophila Kc cells were determined using cloned cDNA fragments. These cDNAs represent poly(A) RNA sequences of different abundance in the cytoplasm of Kc cells, but their steady state concentrations in poly(A) hnRNA was always lower. Of ten different sequences analysed, eight showed some four-fold lower concentration in hnRNA mRNA, two were underrepresented in hnRNA relative to the others. The obvious clustering of mRNA/hnRNA ratios is discussed in relation to sequence complexity and turnover rates of these RNA populations.
利用克隆的cDNA片段测定了果蝇Kc细胞的多聚腺苷酸核RNA和细胞质RNA群体中单个RNA序列的稳态浓度。这些cDNA代表Kc细胞细胞质中不同丰度的多聚腺苷酸RNA序列，但它们在多聚腺苷酸核内不均一RNA中的稳态浓度总是较低。在分析的10个不同序列中，8个在核内不均一RNA/信使RNA中的浓度低约四倍，2个在核内不均一RNA中的含量相对于其他序列较少。结合这些RNA群体的序列复杂性和周转率，讨论了信使RNA/核内不均一RNA比率的明显聚类情况。
