果蝇细胞中总核RNA和聚腺苷酸化核RNA的序列多样性。
Diversity of sequences in total and polyadenylated nuclear RNA from Drosophila cells.
作者信息
Levy B, Johnson C B, McCarthy B J
出版信息
Nucleic Acids Res. 1976 Jul;3(7):1777-89. doi: 10.1093/nar/3.7.1777.
Abstract
Complementary DNA was synthesized using polyadenylated nuclear RNA of cultured Drosophila cells as template. The kinetics of hybridization of this cDNA with nuclear RNA indicated that the complexity of this RNA population is five to ten times greater than that of cytoplasmic mRNA. The same difference in the fraction of DNA represented was obtained when nuclear and cytoplasmic RNA were hybridized with labeled unique sequence DNA. The fraction of the DNA sequences represented in total number of polyadenylated nuclear RNA is much higher than that represented in cytoplasmic RNA.
摘要
以培养的果蝇细胞的聚腺苷酸化核RNA为模板合成互补DNA。该cDNA与核RNA杂交的动力学表明，该RNA群体的复杂性比细胞质mRNA的复杂性大五到十倍。当核RNA和细胞质RNA与标记的单一序列DNA杂交时，在代表的DNA部分也得到了相同的差异。聚腺苷酸化核RNA总数中所代表的DNA序列部分远高于细胞质RNA中所代表的部分。
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