Wong Rebecca S Y, Chow Anthony W
Division of Infectious Diseases, Vancouver Hospital Health Sciencs Center, BC, Canada.
FEMS Microbiol Lett. 2002 Jan 2;206(1):107-13. doi: 10.1111/j.1574-6968.2002.tb10994.x.
A highly specific and reproducible approach for the simultaneous detection of enteric pathogenic bacteria was developed using bacterial hsp60 gene and molecular biological tools. A single pair of universal primers was derived from the highly conserved sequence of hsp60 genes encompassing a 600-bp hypervariable region. PCR amplification followed by either dot blot hybridization or restriction enzyme digestion performed on 38 enteric bacteria indicated that this approach could differentiate not only different genera such as Campylobacter, Yersinia and Vibrio, but also species that are closely related genetically, such as between C. jejuni and C. coli, or between Salmonella and Shigella or Escherichia coli.
利用细菌hsp60基因和分子生物学工具，开发了一种用于同时检测肠道病原菌的高度特异性和可重复性方法。从hsp60基因的高度保守序列中衍生出一对通用引物，该序列包含一个600bp的高变区。对38种肠道细菌进行PCR扩增，然后进行斑点杂交或限制性酶切，结果表明该方法不仅可以区分不同的属，如弯曲杆菌属、耶尔森菌属和弧菌属，还可以区分遗传关系密切的物种，如空肠弯曲菌和大肠弯曲菌之间，或沙门氏菌、志贺氏菌和大肠杆菌之间。
