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圈养黑猩猩粪便中物种的分离与鉴定

Isolation and identification of species from feces of captive chimpanzees.

作者信息

Nomoto Ryohei, Takano Shintaro, Tanaka Kosei, Tsujikawa Yuji, Kusunoki Hiroshi, Osawa Ro

机构信息

Department of Bioresource Science, Organization of Advanced Science and Technology, Kobe University, 1-1 Rokko-dai, Nada-ku, Kobe 657-8501, Japan.

Health Bioscience Team, Organization of Advanced Science and Technology, Kobe University, 1-1 Rokko-dai, Nada-ku, Kobe 657-8501, Japan.

出版信息

Biosci Microbiota Food Health. 2017;36(3):91-99. doi: 10.12938/bmfh.16-027. Epub 2017 Apr 3.

DOI:10.12938/bmfh.16-027
PMID:28748130
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5510154/
Abstract

Recently, gut-dwelling bifidobacteria from chimpanzees, which are phylogenetically close to humans and have feeding habits similar to humans, have been frequently investigated. Given this, we speculated that like humans, chimpanzees would have a unique diversity of bifidobacteria. We herein describe a taxonomically novel member of bifidobacteria isolated from fecal samples of captive chimpanzees. Bifidobacteria were detected in all fecal samples by quantitative polymerase chain reaction. A -like species, which could not be detected using -specific primers targeting the gene sequence, was dominant in the feces of five chimpanzees. Seven bifidobacterial strains were isolated from this group of five chimpanzees, and all isolates were identified as . has previously often been isolated from non-primate animals as well as humans; however, here we demonstrate its presence in a nonhuman primate species.

摘要

最近,人们频繁地对来自黑猩猩的肠道双歧杆菌进行研究,黑猩猩在系统发育上与人类接近,且具有与人类相似的饮食习惯。鉴于此,我们推测,与人类一样,黑猩猩也会有独特的双歧杆菌多样性。我们在此描述了从圈养黑猩猩粪便样本中分离出的一种分类学上的新型双歧杆菌成员。通过定量聚合酶链反应在所有粪便样本中检测到了双歧杆菌。一种无法使用针对 基因序列的特异性引物检测到的类似A的物种,在五只黑猩猩的粪便中占主导地位。从这五只黑猩猩中分离出了七株双歧杆菌菌株,所有分离株均被鉴定为 。 此前经常从非灵长类动物以及人类中分离得到;然而,在这里我们证明了它在非人类灵长类物种中的存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89b4/5510154/6650cf591d98/bmfh-36-091-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89b4/5510154/6650cf591d98/bmfh-36-091-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89b4/5510154/6650cf591d98/bmfh-36-091-g001.jpg

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Genome Announc. 2015 Jan 22;3(1):e01469-14. doi: 10.1128/genomeA.01469-14.
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Biosynthesis and cellular content of folate in bifidobacteria across host species with different diets.不同饮食宿主物种中双歧杆菌叶酸的生物合成及细胞含量
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Establishment of intestinal bacteriology.建立肠道细菌学。
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Mother-to-infant transmission of intestinal bifidobacterial strains has an impact on the early development of vaginally delivered infant's microbiota.母婴传播的肠道双歧杆菌菌株对阴道分娩婴儿早期微生物群的发展有影响。
PLoS One. 2013 Nov 14;8(11):e78331. doi: 10.1371/journal.pone.0078331. eCollection 2013.
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