Liu Alvin Y, True Lawrence D
Department of Urology, University of Washington, Seattle, Washington 98195, USA.
Am J Pathol. 2002 Jan;160(1):37-43. doi: 10.1016/S0002-9440(10)64346-5.
A set of monoclonal antibodies raised against lymphocyte cell surface molecules, the cluster designation (CD) antigens, was used to distinguish the constituent cell types of the prostate. The luminal secretory epithelial, basal epithelial, fibromuscular stromal, nerve sheath, and endothelial cells express distinctive complements of cell surface molecules that were identified by immunohistochemistry using 152 commercially available antibodies. Many of the CD antibodies stained lymphocyte populations in the prostate. These lymphocyte populations were grouped into abundance classes of rare, moderate, and high. Some of these molecules are expressed by multiple cell types, both parenchymal and lymphoid; others are expressed by only one cell type. Distinctive patterns of CD expression, which are most similar to the expression pattern of prostate luminal cells, also characterize a small series of Gleason score 6 prostate cancers. The cell-type specificity of CD molecules increases the prospect of isolating specific cell populations, using such techniques as laser capture microdissection and flow cytometry, for cell-specific molecular studies.
一组针对淋巴细胞细胞表面分子（即分化群（CD）抗原）产生的单克隆抗体被用于区分前列腺的组成细胞类型。管腔分泌上皮细胞、基底上皮细胞、纤维肌基质细胞、神经鞘细胞和内皮细胞表达独特的细胞表面分子互补物，这些分子通过使用152种市售抗体的免疫组织化学方法得以鉴定。许多CD抗体对前列腺中的淋巴细胞群体进行了染色。这些淋巴细胞群体被分为稀少、中等和丰富三类。其中一些分子由多种细胞类型表达，包括实质细胞和淋巴细胞；其他分子仅由一种细胞类型表达。与前列腺管腔细胞表达模式最相似的独特CD表达模式，也表现在一小部分 Gleason 评分6级的前列腺癌中。CD分子的细胞类型特异性增加了使用激光捕获显微切割和流式细胞术等技术分离特定细胞群体以进行细胞特异性分子研究的可能性。
