Branch D W, Dudley D J, LaMarche S, Mitchell M D
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Prostaglandins Leukot Essent Fatty Acids. 1992 Mar;45(3):191-5. doi: 10.1016/0952-3278(92)90112-v.
A relative decrease in endothelial cell prostacyclin production may be pivotal in the genesis of preeclampsia. We determined the effect of sera from preeclamptic women on prostacyclin production by monolayers of normal term human umbilical vein endothelial cells. Endothelial cells were incubated with media containing serum from patients with preeclampsia, non-hypertensive, gestational age-matched pregnant controls, or normal non-pregnant controls (N = 7, all groups). 6-Keto-prostaglandin F1 alpha, the stable metabolite of prostacyclin, was measured directly in the culture medium by radioimmunoassay. Treatment with preeclamptic sera, when associated with a statistically significant increase in prostacyclin metabolite production by endothelial cells. Thus, sera from women with preeclampsia stimulate rather than inhibit prostacyclin production by endothelial cells. We speculate that there is a factor in the sera of women with preeclampsia that functions to activate endothelial cells or which may play a role in the homeostatic mechanisms to balance reduced prostacyclin output in preeclampsia.
内皮细胞前列环素生成的相对减少可能在子痫前期的发病机制中起关键作用。我们测定了子痫前期女性血清对正常足月人脐静脉内皮细胞单层前列环素生成的影响。将内皮细胞与含有子痫前期患者、非高血压且孕周匹配的妊娠对照者或正常非妊娠对照者血清的培养基一起孵育（每组N = 7）。通过放射免疫测定法直接在培养基中测量前列环素的稳定代谢产物6-酮-前列腺素F1α。当与内皮细胞前列环素代谢产物生成的统计学显著增加相关时，用子痫前期血清进行处理。因此，子痫前期女性的血清刺激而非抑制内皮细胞的前列环素生成。我们推测，子痫前期女性血清中存在一种因子，其作用是激活内皮细胞，或者可能在平衡子痫前期前列环素输出减少的稳态机制中发挥作用。
