Sherman N E, Stefansson B, Fox J W, Goldberg J B
Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA.
Dis Markers. 2001;17(4):285-93. doi: 10.1155/2001/653870.
Pseudomonas aeruginosa is a Gram-negative bacterium that is ubiquitous in the environment and can cause a variety of diseases in compromised patients. The genome of P. aeruginosa strain PAO1 has been reported to contain 5570 potential proteins. The value of this genomic database is that new proteins can be recognized to use as diagnostic markers, novel drug targets, and to better understand the physiology of this organism. However, similar to what has been observed in other sequenced bacterial genomes, approximately one third of the potential proteins have no known function. This is somewhat surprising given the long-standing interest in P. aeruginosa as an opportunistic pathogen. Obviously new tools, in addition to sequence similarity analysis, are needed to determine the role of these proteins. Proteomics using two-dimensional gel electrophoresis followed by mass spectrometry to detect and identify P. aeruginosa proteins represents a novel approach to address this gap.
铜绿假单胞菌是一种革兰氏阴性菌，在环境中广泛存在，可在免疫功能低下的患者中引发多种疾病。据报道，铜绿假单胞菌PAO1菌株的基因组包含5570种潜在蛋白质。这个基因组数据库的价值在于，可以识别新的蛋白质，将其用作诊断标志物、新型药物靶点，并更好地了解该生物体的生理学特性。然而，与其他已测序的细菌基因组中观察到的情况类似，大约三分之一的潜在蛋白质尚无已知功能。鉴于长期以来对铜绿假单胞菌作为机会致病菌的关注，这多少有些令人惊讶。显然，除了序列相似性分析之外，还需要新的工具来确定这些蛋白质的作用。使用二维凝胶电泳随后进行质谱分析来检测和鉴定铜绿假单胞菌蛋白质的蛋白质组学，代表了一种填补这一空白的新方法。
