Hoff H F, Heideman C L, Noon G P, Meyer J S
Stroke. 1975 Sep-Oct;6(5):531-4. doi: 10.1161/01.str.6.5.531.
Apoproteins from plasma lipoproteins were localized by immunofluorescence techniques in human carotid artery atherosclerotic lesions. These studies were performed in light of the possible importance of these apoproteins in both lipid metabolism and the pathogenesis of atherosclerosis. ApoA-I from high density lipoproteins, apoB from low density lipoproteins, and apoC-III from very low density lipoproteins were localized also as markers for their respective lipoproteins, since the latter cross-react immunologically. The three apoproteins were localized to the same regions of lesions as neutral lipids and, to some extent, fibrinogen. These regions consisted of bands of collagen fibers, usually deeper within the lesion, and the lipid core or atheroma of such advanced lesions. Although the superposition of localization for the three apoproteins and lipid was only 53%, it was suggested that deviation from complete superposition was due to the abrupt changes in lesion structure resulting from the focal nature of the atherosclerotic process. These results suggest that there is a broader specificity than previously implied of the interaction between such lesion components as connective tissue and extracellular lipid accumulations, and apoproteins from plasma lipoproteins. This interaction is believed to result in a net retention within atherosclerotic lesions of human extracranial arteries of these plasma-derived factors, either as free apoproteins or as native lipoproteins.
利用免疫荧光技术在人类颈动脉粥样硬化病变中对血浆脂蛋白中的载脂蛋白进行了定位。鉴于这些载脂蛋白在脂质代谢和动脉粥样硬化发病机制中可能具有的重要性，开展了这些研究。高密度脂蛋白中的载脂蛋白A-I、低密度脂蛋白中的载脂蛋白B以及极低密度脂蛋白中的载脂蛋白C-III也被定位，作为各自脂蛋白的标志物，因为后者存在免疫交叉反应。这三种载脂蛋白与中性脂质以及在一定程度上与纤维蛋白原定位于病变的相同区域。这些区域由胶原纤维带组成，通常位于病变较深处，以及此类晚期病变的脂质核心或粥样瘤。尽管三种载脂蛋白和脂质的定位叠加率仅为53%，但有人认为定位偏离完全叠加是由于动脉粥样硬化过程的局灶性导致病变结构的突然变化。这些结果表明，结缔组织和细胞外脂质积聚等病变成分与血浆脂蛋白中的载脂蛋白之间的相互作用具有比先前认为的更广泛的特异性。据信这种相互作用会导致这些源自血浆的因子以游离载脂蛋白或天然脂蛋白的形式在人类颅外动脉的动脉粥样硬化病变中净潴留。
