Hoff H F, Heideman C L, Gaubatz J W
Stroke. 1977 May-Jun;8(3):366-70. doi: 10.1161/01.str.8.3.366.
Low (LDL) and very low (VLDL) density lipoproteins retained in grossly normal and atherosclerotic human intracranial arteries have been quantitated using an electro-immunoassay directed against apolipoprotein B (apoB), the major protein of these two lipoprotein fractions. Buffer-homogenates of grossly normal arteries contained apoB amounts ranging from less than 0.04 to 1.58 microng/mg tissue dry weight, while those of atherosclerotic plaques gave values ranging from 0.80 to 3.9 microng per mg tissue dry weight. These results were consistent with immunofluorescence studies localizing apoB in these arteries. Plaques also contained a remaining fraction of tightly-bound apoB as evidenced by positive immunofluorescence in sections of pellets from buffer homogenates. This was in contrast to the negative results from grossly normal arteries. These results would suggest that retention of apoB by intracranial arteries correlates positively with vessel lesions. Arterial apoB is present in both grossly normal regions and plaques in a loosely-bound form, possibly representing intact lipoprotein. ApoB is also present in a tightly-bound form in plaques.
利用针对载脂蛋白B（apoB）的免疫电泳法，对大体正常和动脉粥样硬化的人颅内动脉中留存的低密度（LDL）和极低密度（VLDL）脂蛋白进行了定量分析，apoB是这两种脂蛋白组分的主要蛋白质。大体正常动脉的缓冲液匀浆中，apoB含量范围为每毫克组织干重小于0.04至1.58微克，而动脉粥样硬化斑块的缓冲液匀浆中，每毫克组织干重的apoB含量范围为0.80至3.9微克。这些结果与将apoB定位在这些动脉中的免疫荧光研究结果一致。斑块中还含有一部分紧密结合的apoB，缓冲液匀浆沉淀切片中的阳性免疫荧光证明了这一点。这与大体正常动脉的阴性结果形成对比。这些结果表明，颅内动脉对apoB的留存与血管病变呈正相关。动脉apoB以松散结合的形式存在于大体正常区域和斑块中，可能代表完整的脂蛋白。apoB在斑块中也以紧密结合的形式存在。
