影响异亮氨酰 - tRNA合成酶罗斯曼折叠的突变与金黄色葡萄球菌的低水平莫匹罗星耐药性相关。
Mutations affecting the Rossman fold of isoleucyl-tRNA synthetase are correlated with low-level mupirocin resistance in Staphylococcus aureus.
作者信息
Antonio Martin, McFerran Neil, Pallen Mark J
机构信息
Microbial Genomics and Pathogenesis Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, BT15 2TT, United Kingdom.
出版信息
Antimicrob Agents Chemother. 2002 Feb;46(2):438-42. doi: 10.1128/AAC.46.2.438-442.2002.
Abstract
The isoleucyl-tRNA synthetase (ileS) gene was sequenced in toto from 9 and in part from 31 Staphylococcus aureus strains with various degrees of susceptibility to mupirocin. All strains for which the mupirocin MIC was greater than 8 microg/ml contained point mutations affecting the Rossman fold via Val-to-Phe changes at either residue 588 (V588F) or residue 631 (V631F). The importance of the V588F mutation was confirmed by an allele-specific PCR survey of 32 additional strains. Additional mutations of uncertain significance were found in residues clustered on the surface of the IleS protein.
摘要
对9株对莫匹罗星具有不同敏感性的金黄色葡萄球菌菌株的异亮氨酰 - tRNA合成酶（ileS）基因进行了全序列测定，并对另外31株进行了部分序列测定。所有莫匹罗星MIC大于8μg/ml的菌株都含有点突变，这些突变通过588位残基（V588F）或631位残基（V631F）的缬氨酸到苯丙氨酸的变化影响罗斯曼折叠。通过对另外32株菌株的等位基因特异性PCR检测证实了V588F突变的重要性。在IleS蛋白表面聚集的残基中发现了其他意义不确定的突变。
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