从昆士兰临床犬类皮肤和耳部样本中培养出的金黄色葡萄球菌、中间葡萄球菌和凝固酶阴性葡萄球菌的分子流行病学特征

Molecular Epidemiological Characteristics of , and Coagulase-Negative Staphylococci Cultured from Clinical Canine Skin and Ear Samples in Queensland.

作者信息

Horsman Sara, Zaugg Julian, Meler Erika, Mikkelsen Deirdre, Soares Magalhães Ricardo J, Gibson Justine S

机构信息

School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia.

Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.

出版信息

Antibiotics (Basel). 2025 Jan 13;14(1):80. doi: 10.3390/antibiotics14010080.

DOI:10.3390/antibiotics14010080

PMID:39858366

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11761246/

Abstract

Infections in dogs caused by methicillin-resistant staphylococci (MRS) present limited treatment options. This study's objective was to investigate the molecular epidemiology of spp. cultured exclusively from clinical canine skin and ear samples in Queensland, Australia, using whole-genome sequencing (WGS). Forty-two spp. isolated from clinical canine skin and ear samples, from an unknown number of dogs, were sourced from two veterinary diagnostic laboratories between January 2022 and May 2023. These isolates underwent matrix-assisted laser desorption ionisation- time of flight bacterial identification, minimum inhibitory concentration testing using Sensititre plates and WGS. Phylogenetic trees and core genome multilocus sequence typing (cgMLST) minimum spanning trees (MSTs) were constructed. The isolates included methicillin-resistant and -sensitive (MRSP: 57.1%, 24/42; and MSSP: 19.1%, 8/42), methicillin-resistant and -sensitive (MRSC: 14.3%, 6/42; and MSSC: 2.4%, 1/42) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS: 7.1%, 3/42). Thirty-nine isolates were included after WGS, where all MRS harboured the gene. Eighteen sequence types (STs) were identified, including three novel MRSP and six novel MSSP STs. MRSP ST496-V-VII (23%; 9/39) and MRSP ST749-IV-(IVg) (12.8%; 5/39) were commonly isolated. Phylogenetic analysis of single nucleotide polymorphisms showed that MRSP, MRSC and MSSC were similar to globally isolated staphylococci from canine skin and ear infections. Using cgMLST MSTs, MRSP isolates were not closely related to global strains. Our findings revealed a genotypically diverse geographical distribution and phylogenetic relatedness of staphylococci cultured from clinical canine skin and ear samples across Queensland. This highlights the importance of ongoing surveillance to aid in evidence-based treatment decisions and antimicrobial stewardship.

摘要

耐甲氧西林葡萄球菌（MRS）引起的犬类感染的治疗选择有限。本研究的目的是利用全基因组测序（WGS）调查仅从澳大利亚昆士兰州临床犬皮肤和耳部样本中培养出的葡萄球菌属的分子流行病学。2022年1月至2023年5月期间，从两个兽医诊断实验室获取了42株从临床犬皮肤和耳部样本中分离出的葡萄球菌属菌株，这些样本来自数量不明的犬只。这些分离株进行了基质辅助激光解吸电离飞行时间细菌鉴定、使用Sensititre平板的最低抑菌浓度测试和WGS。构建了系统发育树和核心基因组多位点序列分型（cgMLST）最小生成树（MST）。分离株包括耐甲氧西林和对甲氧西林敏感的葡萄球菌（MRSP：57.1%，24/42；MSSP：19.1%，8/42）、耐甲氧西林和对甲氧西林敏感的中间葡萄球菌（MRSC：14.3%，6/42；MSSC：2.4%，1/42）以及耐甲氧西林凝固酶阴性葡萄球菌（MR-CoNS：7.1%，3/42）。WGS后纳入39株分离株，所有MRS均携带mecA基因。鉴定出18种序列类型（ST），包括3种新型MRSP和6种新型MSSP ST。常见分离出的是MRSP ST496-V-VII（23%；9/39）和MRSP ST749-IV-(IVg)（12.8%；5/39）。单核苷酸多态性的系统发育分析表明，MRSP、MRSC和MSSC与全球从犬皮肤和耳部感染中分离出的葡萄球菌相似。使用cgMLST MST，MRSP分离株与全球菌株关系不密切。我们的研究结果揭示了昆士兰州临床犬皮肤和耳部样本中培养出的葡萄球菌在基因型上的多样地理分布和系统发育相关性。这突出了持续监测对于辅助基于证据的治疗决策和抗菌药物管理的重要性。