• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
DNA adenine methylase overproduction in Yersinia pseudotuberculosis alters YopE expression and secretion and host immune responses to infection.假结核耶尔森菌中DNA腺嘌呤甲基化酶的过量表达会改变YopE的表达与分泌以及宿主对感染的免疫反应。
Infect Immun. 2002 Feb;70(2):1006-9. doi: 10.1128/IAI.70.2.1006-1009.2002.
2
LcrV synthesis is altered by DNA adenine methylase overproduction in Yersinia pseudotuberculosis and is required to confer immunity in vaccinated hosts.在假结核耶尔森氏菌中，DNA腺嘌呤甲基化酶过量表达会改变LcrV的合成，而LcrV的合成对于在接种疫苗的宿主体内产生免疫是必需的。
Infect Immun. 2004 Nov;72(11):6707-10. doi: 10.1128/IAI.72.11.6707-6710.2004.
3
DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae.DNA 腺嘌呤甲基化酶对生存能力至关重要，并且在假结核耶尔森菌和霍乱弧菌的发病机制中发挥作用。
Infect Immun. 2001 Dec;69(12):7610-5. doi: 10.1128/IAI.69.12.7610-7615.2001.
4
The proinflammatory response induced by wild-type Yersinia pseudotuberculosis infection inhibits survival of yop mutants in the gastrointestinal tract and Peyer's patches.野生型假结核耶尔森菌感染诱导的促炎反应会抑制yop突变体在胃肠道和派伊尔结中的存活。
Infect Immun. 2006 Mar;74(3):1516-27. doi: 10.1128/IAI.74.3.1516-1527.2006.
5
A protective epitope in type III effector YopE is a major CD8 T cell antigen during primary infection with Yersinia pseudotuberculosis.III 型效应物 YopE 中的保护性表位是初期感染假结核耶尔森菌时主要的 CD8 T 细胞抗原。
Infect Immun. 2012 Jan;80(1):206-14. doi: 10.1128/IAI.05971-11. Epub 2011 Nov 7.
6
Immunization with a DNA adenine methylase over-producing Yersinia pseudotuberculosis vaccine confers robust cross-protection against heterologous pathogenic serotypes.用产生大量腺嘌呤甲基化酶的 DNA 免疫接种假结核耶尔森氏菌疫苗可对异源致病性血清型提供强大的交叉保护。
Vaccine. 2014 Mar 14;32(13):1451-9. doi: 10.1016/j.vaccine.2014.01.036. Epub 2014 Feb 6.
7
IscR is essential for yersinia pseudotuberculosis type III secretion and virulence.IscR对于假结核耶尔森菌III型分泌和毒力至关重要。
PLoS Pathog. 2014 Jun 12;10(6):e1004194. doi: 10.1371/journal.ppat.1004194. eCollection 2014 Jun.
8
CCR2+ Inflammatory Dendritic Cells and Translocation of Antigen by Type III Secretion Are Required for the Exceptionally Large CD8+ T Cell Response to the Protective YopE69-77 Epitope during Yersinia Infection.CCR2+炎性树突状细胞以及通过III型分泌进行的抗原转运，对于耶尔森氏菌感染期间针对保护性YopE69-77表位产生的异常强大的CD8+ T细胞应答是必需的。
PLoS Pathog. 2015 Oct 15;11(10):e1005167. doi: 10.1371/journal.ppat.1005167. eCollection 2015 Oct.
9
Influence of Yersinia pseudotuberculosis outer proteins (Yops) on interleukin-12, tumor necrosis factor alpha and nitric oxide production by peritoneal macrophages.假结核耶尔森菌外膜蛋白（Yops）对腹膜巨噬细胞产生白细胞介素-12、肿瘤坏死因子α和一氧化氮的影响。
Immunol Lett. 2004 Jun 15;94(1-2):91-8. doi: 10.1016/j.imlet.2004.04.007.
10
Role of YopK in Yersinia pseudotuberculosis resistance against polymorphonuclear leukocyte defense.YopK 在假结核耶尔森氏菌抵抗多形核白细胞防御中的作用。
Infect Immun. 2013 Jan;81(1):11-22. doi: 10.1128/IAI.00650-12. Epub 2012 Oct 22.

引用本文的文献

1
Moving toward the Inclusion of Epigenomics in Bacterial Genome Evolution: Perspectives and Challenges.迈向将表观基因组学纳入细菌基因组进化研究：观点与挑战
Int J Mol Sci. 2024 Apr 17;25(8):4425. doi: 10.3390/ijms25084425.
2
Diverse Roles for a Conserved DNA-Methyltransferase in the Entomopathogenic Bacterium .一种保守的 DNA 甲基转移酶在昆虫病原细菌中的多种作用。
Int J Mol Sci. 2022 Oct 9;23(19):11981. doi: 10.3390/ijms231911981.
3
Novel Identification of Bacterial Epigenetic Regulations Would Benefit From a Better Exploitation of Methylomic Data.更好地利用甲基化组数据将有助于细菌表观遗传调控的新鉴定。
Front Microbiol. 2021 May 14;12:685670. doi: 10.3389/fmicb.2021.685670. eCollection 2021.
4
Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts.在与无脊椎动物宿主相互作用过程中 Photorhabdus Dam 甲基转移酶的作用。
PLoS One. 2019 Oct 9;14(10):e0212655. doi: 10.1371/journal.pone.0212655. eCollection 2019.
5
The complete methylome of an entomopathogenic bacterium reveals the existence of loci with unmethylated Adenines.昆虫病原细菌的全甲基组揭示了存在未甲基化腺嘌呤的基因座。
Sci Rep. 2018 Aug 14;8(1):12091. doi: 10.1038/s41598-018-30620-5.
6
DNA Adenine Methyltransferase (Dam) Overexpression Impairs Motility and Virulence.DNA腺嘌呤甲基转移酶（Dam）过表达会损害运动性和毒力。
Front Microbiol. 2017 Sep 1;8:1671. doi: 10.3389/fmicb.2017.01671. eCollection 2017.
7
The dam replacing gene product enhances Neisseria gonorrhoeae FA1090 viability and biofilm formation.替代基因产物的水坝增强了淋病奈瑟菌FA1090的活力和生物膜形成。
Front Microbiol. 2014 Dec 17;5:712. doi: 10.3389/fmicb.2014.00712. eCollection 2014.
8
The methylome of the gut microbiome: disparate Dam methylation patterns in intestinal Bacteroides dorei.肠道微生物组的甲基组：肠道拟杆菌中不同的 Dam 甲基化模式。
Front Microbiol. 2014 Jul 17;5:361. doi: 10.3389/fmicb.2014.00361. eCollection 2014.
9
Inhibition of Yersinia pestis DNA adenine methyltransferase in vitro by a stibonic acid compound: identification of a potential novel class of antimicrobial agents.抑制鼠疫耶尔森氏菌 DNA 腺嘌呤甲基转移酶在体外的一个亚砷酸化合物：鉴定潜在的新型抗菌药物。
Br J Pharmacol. 2013 Jan;168(1):172-88. doi: 10.1111/j.1476-5381.2012.02134.x.
10
The phasevarion: phase variation of type III DNA methyltransferases controls coordinated switching in multiple genes.相变异体：III 型 DNA 甲基转移酶的相变异控制多个基因的协调开关。
Nat Rev Microbiol. 2010 Mar;8(3):196-206. doi: 10.1038/nrmicro2283. Epub 2010 Feb 8.

本文引用的文献

1
Salmonella DNA adenine methylase mutants elicit protective immune responses to homologous and heterologous serovars in chickens.沙门氏菌DNA腺嘌呤甲基化酶突变体在鸡体内引发对同源和异源血清型的保护性免疫反应。
Infect Immun. 2001 Dec;69(12):7950-4. doi: 10.1128/IAI.69.12.7950-7954.2001.
2
DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae.DNA 腺嘌呤甲基化酶对生存能力至关重要，并且在假结核耶尔森菌和霍乱弧菌的发病机制中发挥作用。
Infect Immun. 2001 Dec;69(12):7610-5. doi: 10.1128/IAI.69.12.7610-7615.2001.
3
Roles of DNA adenine methylation in regulating bacterial gene expression and virulence.DNA腺嘌呤甲基化在调控细菌基因表达和毒力中的作用。
Infect Immun. 2001 Dec;69(12):7197-204. doi: 10.1128/IAI.69.12.7197-7204.2001.
4
Salmonella DNA adenine methylase mutants confer cross-protective immunity.沙门氏菌DNA腺嘌呤甲基化酶突变体赋予交叉保护性免疫。
Infect Immun. 2001 Nov;69(11):6725-30. doi: 10.1128/IAI.69.11.6725-6730.2001.
5
YopE of Yersinia, a GAP for Rho GTPases, selectively modulates Rac-dependent actin structures in endothelial cells.耶尔森氏菌的YopE蛋白，一种Rho GTP酶激活蛋白，可选择性地调节内皮细胞中依赖Rac的肌动蛋白结构。
Cell Microbiol. 2001 May;3(5):301-10. doi: 10.1046/j.1462-5822.2001.00114.x.
6
How to survive in the host: the Yersinia lesson.如何在宿主体内生存：耶尔森氏菌的启示。
Microbes Infect. 2000 Oct;2(12):1451-60. doi: 10.1016/s1286-4579(00)01300-9.
7
The RhoGAP activity of the Yersinia pseudotuberculosis cytotoxin YopE is required for antiphagocytic function and virulence.假结核耶尔森菌细胞毒素YopE的RhoGAP活性是抗吞噬功能和毒力所必需的。
Mol Microbiol. 2000 Aug;37(3):515-27. doi: 10.1046/j.1365-2958.2000.02021.x.
8
A chromosomally encoded type III secretion pathway in Yersinia enterocolitica is important in virulence.小肠结肠炎耶尔森菌中由染色体编码的III型分泌途径在毒力方面很重要。
Mol Microbiol. 2000 Jun;36(6):1436-46. doi: 10.1046/j.1365-2958.2000.01964.x.
9
Molecular and cell biology aspects of plague.鼠疫的分子与细胞生物学方面
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8778-83. doi: 10.1073/pnas.97.16.8778.
10
Type III secretion: a bacterial device for close combat with cells of their eukaryotic host.III型分泌系统：细菌与真核宿主细胞近距离作战的一种机制
Philos Trans R Soc Lond B Biol Sci. 2000 May 29;355(1397):681-93. doi: 10.1098/rstb.2000.0608.

假结核耶尔森菌中DNA腺嘌呤甲基化酶的过量表达会改变YopE的表达与分泌以及宿主对感染的免疫反应。

DNA adenine methylase overproduction in Yersinia pseudotuberculosis alters YopE expression and secretion and host immune responses to infection.

作者信息

Julio Steven M, Heithoff Douglas M, Sinsheimer Robert L, Low David A, Mahan Michael J

机构信息

Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106, USA.

出版信息

Infect Immun. 2002 Feb;70(2):1006-9. doi: 10.1128/IAI.70.2.1006-1009.2002.

DOI:10.1128/IAI.70.2.1006-1009.2002
PMID:11796641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC127708/
Abstract

Yersinia pseudotuberculosis mutants that overproduce the DNA adenine methylase (Dam) are highly attenuated, confer fully protective immune responses, and secrete several Yersinia virulence proteins (Yersinia outer proteins [Yops]) under conditions that are nonpermissive for secretion in wild-type strains. We examined here the effects of Dam overproduction on Yersinia virulence determinant expression and secretion, as well as the host immune response to Yersinia antigens. Western blot analysis with convalescent antisera identified several low-calcium-responsive antigens whose synthesis was affected by Dam overproduction. One of these antigens was shown to be the type III secretion effector protein, YopE, a cytotoxin involved in antiphagocytosis. Dam overproduction disrupted both the thermal and calcium regulation of YopE synthesis and relaxed the thermal but not the calcium dependence of YopE secretion. Altered expression and/or secretion of Yersinia proteins in Dam-overproducing strains may contribute to the decreased virulence and heightened immunity observed in vaccinated hosts and may provide a means by which to deliver heterologous antigens and/or immune modulators of the inflammatory response.

摘要

过量产生DNA腺嘌呤甲基化酶（Dam）的假结核耶尔森菌突变体高度减毒，能引发完全保护性免疫反应，并且在野生型菌株不允许分泌的条件下分泌几种耶尔森菌毒力蛋白（耶尔森菌外膜蛋白[Yops]）。我们在此研究了过量产生Dam对耶尔森菌毒力决定因素表达和分泌的影响，以及宿主对耶尔森菌抗原的免疫反应。用恢复期抗血清进行的蛋白质印迹分析鉴定出几种低钙反应性抗原，其合成受过量产生Dam的影响。其中一种抗原被证明是III型分泌效应蛋白YopE，一种参与抗吞噬作用的细胞毒素。过量产生Dam破坏了YopE合成的温度和钙调节，并且放宽了YopE分泌的温度依赖性，但未改变其钙依赖性。在过量产生Dam的菌株中，耶尔森菌蛋白表达和/或分泌的改变可能导致在接种疫苗的宿主中观察到的毒力降低和免疫力增强，并且可能提供一种递送异源抗原和/或炎症反应免疫调节剂的方法。