Haid Clemens, Kaps Simone, Gönczi Enikö, Hässig Michael, Metzler Alfred, Spiess Bernhard M, Richter Marianne
Department of Small Animals, Ophthalmology Unit, Vetsuisse Faculty, University of Zürich, Winterhurerstrasse 260, 8057 Zürich, Switzerland.
Vet Ophthalmol. 2007 Sep-Oct;10(5):278-84. doi: 10.1111/j.1463-5224.2007.00550.x.
Recombinant feline interferon omega (rFeIFN-omega), a type I IFN, may have the potential to limit virus replication and associated clinical signs when administered early on in the course of feline herpesvirus type 1 (FHV-1) infection and reactivation, respectively. The effect of rFeIFN-omega pretreatment on the course of subsequent FHV-1 infection in cats was investigated.
Nine SPF cats were divided into an IFN group (n = 5) and a control-group (n = 4).
The IFN group was pretreated for 2 days with 10 000 units rFeIFN-omega twice a day topically into both eyes and 20 000 units rFeIFN-omega once a day orally, whereas the control group was mock-treated. Subsequently all cats were infected with FHV-1. Samples for FHV-1 DNA detection and quantitation, virus isolation, and titration of FHV-1 antibodies were collected. Clinical and ocular signs were recorded and scored.
Courses of median individual clinical and ocular scores and virus load did not differ significantly between both groups using anova for repeated measurements. Analysis (anova) of each individual ocular parameter revealed significantly high scores for epithelial keratitis (P = 0.016) in the IFN group compared to the control group. Periods of virus shedding did not differ significantly between both groups using the Wilcoxon rank sum test.
Results indicated a lack of beneficial effects of rFeIFN-omega pretreatment in the course of primary FHV-1 infection in cats.
重组猫ω干扰素（rFeIFN-ω），一种I型干扰素，在猫疱疹病毒1型（FHV-1）感染及再激活过程中早期给药时，可能具有限制病毒复制及相关临床症状的潜力。研究了rFeIFN-ω预处理对猫后续FHV-1感染病程的影响。
9只无特定病原体（SPF）猫被分为干扰素组（n = 5）和对照组（n = 4）。
干扰素组用10000单位rFeIFN-ω每天两次局部滴眼，持续2天，并每天一次口服20000单位rFeIFN-ω进行预处理，而对照组进行模拟处理。随后所有猫均感染FHV-1。收集用于FHV-1 DNA检测、定量、病毒分离及FHV-1抗体滴定的样本。记录并评分临床及眼部症状。
使用重复测量方差分析，两组之间的个体临床和眼部评分中位数及病毒载量病程无显著差异。对每个个体眼部参数进行分析（方差分析）显示，与对照组相比，干扰素组上皮性角膜炎的评分显著较高（P = 0.016）。使用Wilcoxon秩和检验，两组之间的病毒脱落期无显著差异。
结果表明rFeIFN-ω预处理对猫原发性FHV-1感染病程缺乏有益作用。
