Liu X, Yu T, Zhao M, Tang X, Gu Q, Liu N
Department of Internal Medicine, First Teaching Hospital of Beijing Medical University, Beijing 100034.
Zhonghua Nei Ke Za Zhi. 1999 Jul;38(7):451-4.
To inquire into the diagnostic significance of antineutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis (UC).
Serum ANCA from 58 UC patients, 43 non-UC patients and 58 healthy donors were detected with indirect immunofluorescence (IIF), enzyme linked immunosorbent assay (ELISA)and Western blot.
The sensitivity and specificity of the presence of ANCA in the diagnosis of UC were 37.93% and 100% respectively. In the group of UC, patients with mild, moderate and severe clinical manifestations had positive ANCA rates of 17.65%, 41.67% and 52.94% respectively. The occurrence rates of mucosal vasculitis and grade III-V mucosal inflammation were 78.95% and 78.95% respectively in ANCA-positive group, but 37.04% and 44.44% in ANCA-negative group. The levels of the binding of the five kinds of ANCA antigens i.e. myeloperoxidase (MPO), bactericidal/permeability increasing protein (BPI), lactoferrin (LF), cathepsin G (CG) and proteinase-3 (PR-3) with the serum of UC patients were 13.99%, 13.79%, 10.34% 10.34% and 8.62% respectively. Specific protein strips were observed in 48.28% of the UC patients with Western blot technique, 47,000 polypeptide being the most common and making up 22.41% of them.
The detection of ANCA was an auxiliary method for the diagnosis of UC. At present, UC-associated target antigens were not yet clarified, 47,000 polypeptide may be one of them. ANCA may participate in the pathogenesis of UC.
探讨抗中性粒细胞胞浆抗体（ANCA）在溃疡性结肠炎（UC）中的诊断意义。
采用间接免疫荧光法（IIF）、酶联免疫吸附测定（ELISA）和蛋白质印迹法检测58例UC患者、43例非UC患者及58例健康献血者血清中的ANCA。
ANCA在UC诊断中的敏感性和特异性分别为37.93%和100%。在UC组中，轻度、中度和重度临床表现患者的ANCA阳性率分别为17.65%、41.67%和52.94%。ANCA阳性组黏膜血管炎和Ⅲ - Ⅴ级黏膜炎症的发生率分别为78.95%和78.95%，而ANCA阴性组分别为37.04%和44.44%。UC患者血清与髓过氧化物酶（MPO）、杀菌/通透性增加蛋白（BPI）、乳铁蛋白（LF）、组织蛋白酶G（CG）和蛋白酶 - 3（PR - 3）这五种ANCA抗原的结合水平分别为13.99%、13.79%、10.34%、10.34%和8.62%。采用蛋白质印迹技术在48.28%的UC患者中观察到特异性蛋白条带，其中47000多肽最为常见，占22.41%。
ANCA检测是UC诊断的辅助方法。目前，与UC相关的靶抗原尚未明确，47000多肽可能是其中之一。ANCA可能参与UC的发病机制。
