[小皮盘菌多糖对氧自由基和脂质过氧化的影响]
[The effects of the polysaccharides from Dermatocarpon miniatum on oxygen radicals and lipid peroxidation].
作者信息
Jin J, Bian X, Ge P, Jing H, Ding L, Ding D
机构信息
Xi'an Jiao Tong University, Xi'an 710061.
出版信息
Zhong Yao Cai. 2001 Sep;24(9):660-1.
OBJECTIVE
To study the effects of Dermatocarpon miniatum (DEM) polysaccharides scavenging oxygen radicals and inhibiting lipid peroxidation.
METHODS
.OH was produced by Fenton reaction and O2-. was produced by the oxidation of pyrogallol. The inhibition to LPO was determined by the colorimetry for testing the relative content of MDA.
RESULTS
DEM polysaccharides could scavenge oxygen radical. The amounts of scavenging 50% (EC50) .OH was 1.72 mg/ml, the EC50 for O2-. was 2.12 mg/ml. DEM polysaccharides could also decrease the content of MDA.
CONCLUSION
DEM polysaccharides has the effects of scavenging oxygen radicals and the inhibition to LPO. Its activities showed positive correlation with the amounts.
目的
研究红粉鹿角菜（DEM）多糖清除氧自由基及抑制脂质过氧化的作用。
方法
通过Fenton反应产生·OH，通过邻苯三酚氧化产生O2-。采用比色法测定丙二醛（MDA）的相对含量来确定对脂质过氧化（LPO）的抑制作用。
结果
DEM多糖能够清除氧自由基。清除50%（半数有效浓度，EC50）·OH的量为1.72mg/ml，清除O2-的EC50为2.12mg/ml。DEM多糖还能降低MDA的含量。
结论
DEM多糖具有清除氧自由基及抑制脂质过氧化的作用。其活性与剂量呈正相关。
Zotero 插件