Capek P, Machová E, Turjan J
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, 845 38 Bratislava, Slovakia.
Int J Biol Macromol. 2009 Jan 1;44(1):75-80. doi: 10.1016/j.ijbiomac.2008.10.007. Epub 2008 Oct 30.
Crude polysaccharides, isolated from the aerial parts of sage (Salvia officinalis L.) by sequential extraction with water (A), hot ammonium oxalate (B), dimethyl sulfoxide (C), 1M (D) and 4M (E) potassium hydroxide solutions, and six ion-exchange fractions of A were examined for their ability to inhibit peroxidation of liposome lipid by hydroxyl radicals and to reduced DPPH radical content. The highest inhibition of liposome lipid peroxidation was found with crude polysaccharides A, B and D, antioxidant activities reached approximately 37%. The purified fractions A1 and A2 inhibited the liposome peroxidation to approximately 35%. However, the radical scavenging abilities of the most active crude polysaccharides A, B and C on DPPH radicals were found in the range 80-90%, while the most active purified fractions A3-A6 in three or fourfold doses achieved 75-92%. The least effective tested polysaccharides succeeded 20% inhibition using both methods.
通过依次用水（A）、热草酸铵（B）、二甲基亚砜（C）、1M（D）和4M（E）氢氧化钾溶液从鼠尾草（Salvia officinalis L.）地上部分提取得到粗多糖，并对A的六个离子交换级分进行了检测，考察它们抑制羟基自由基引发的脂质体脂质过氧化以及降低DPPH自由基含量的能力。发现粗多糖A、B和D对脂质体脂质过氧化的抑制作用最强，抗氧化活性约达到37%。纯化级分A1和A2对脂质体过氧化的抑制率约为35%。然而，活性最强的粗多糖A、B和C对DPPH自由基的清除能力在80 - 90%的范围内，而活性最强的纯化级分A3 - A6在三到四倍剂量下的清除率达到75 - 92%。用这两种方法测试，效果最差的多糖的抑制率为20%。
