Valdés R, Córdoba F
Agents Actions. 1975 Feb;5(1):64-8. doi: 10.1007/BF02027162.
Two newly described sesquiterpene lactones, Zexbrevin A and Zexbrevin B, were administered to mice at microgram doses before a single immunizing dose of sheep red blood cells (SRBC), bovine serum albumine (BSA), or Egg albumin (Ea). It was found out that drug treated and immunized animals displayed high numbers of specific spleen rosette forming cells (RFC), as well as elevated titers of circulating antibodies, as compared with mice stimulated solely with antigens. 500 mug of Zexbrevin A and Zexbrevin B was about the best dose for rosette increase; 700 mug or lesser amounts of both drugs induced a decline in the rosette numbers. It was evident that Zexbrevins had to be administered 48 hours before antigen stimulation; no potentiation was obtained if the drugs were given 3 to 11 days before the antigen. No effect was obtained, as well, when mice were treated with drug and antigen on the same day. In the presence of Zexbrevins the radioactive thymidine uptake of cultured normal mouse spleen cells will not increase above controls, indicating that mitogenic effects appear not to be involved in the potentiation. It is concluded that the sesquiterpene lactones exert in mice a potent stimulation of the in vivo response.
在给小鼠单次注射免疫剂量的绵羊红细胞（SRBC）、牛血清白蛋白（BSA）或卵清蛋白（Ea）之前，以微克剂量给小鼠施用两种新描述的倍半萜内酯，即泽布雷文A和泽布雷文B。结果发现，与仅用抗原刺激的小鼠相比，经药物处理并免疫的动物表现出大量的特异性脾玫瑰花结形成细胞（RFC），以及循环抗体滴度升高。500微克的泽布雷文A和泽布雷文B是增加玫瑰花结的最佳剂量；两种药物700微克或更少的量会导致玫瑰花结数量下降。很明显，泽布雷文必须在抗原刺激前48小时给药；如果在抗原前3至11天给药，则无法获得增强作用。当在同一天用药物和抗原处理小鼠时，也没有效果。在存在泽布雷文的情况下，培养的正常小鼠脾细胞的放射性胸苷摄取不会比对照增加，这表明有丝分裂作用似乎不参与增强作用。得出的结论是，倍半萜内酯在小鼠体内对体内反应有强烈的刺激作用。
