在P1无葡萄糖/磷酸盐培养基中增加合成血清替代物（SSS）浓度可提高着床率：一项比较研究。
Increasing synthetic serum substitute (SSS) concentrations in P1 glucose/phosphate-free medium improves implantation rate: a comparative study.
作者信息
Ben-Yosef D, Yovel I, Schwartz T, Azem F, Lessing J B, Amit A
机构信息
The IVF Unit, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Israel.
出版信息
J Assist Reprod Genet. 2001 Nov;18(11):588-92. doi: 10.1023/a:1013156804589.
PURPOSE
To assess the comparative efficacy of IVF medium (MediCult, with 5.2 mM glucose) and a glucose/phosphate-free medium, P1 (Irvine Scientific), and to investigate the influence of increasing the serum supplementation (synthetic serum substitute; SSS; Irvine Scientific) to P1 on embryo development and implantation.
METHODS
Patients were randomly assigned to IVF medium (Group 1, cycles n = 172) or P1 supplemented with 10% SSS (Group 2, cycles n = 229) according to the medium scheduled for use on the day of oocyte retrieval. Another 555 IVF consequent cycles (Group 3) were performed using increased SSS concentrations (20%) in P1 medium.
RESULTS AND CONCLUSION
In this large series of IVF cycles, we herein demonstrate that significantly higher pregnancy and implantation rates were found when embryos were cultured in glucose/phosphate-free medium P1 supplemented with 20% SSS compared to supplementation with the lower SSS concentration and with IVF medium.
目的
评估体外受精培养基（MediCult，含5.2 mM葡萄糖）和无葡萄糖/磷酸盐培养基P1（欧文科学公司）的相对疗效，并研究增加P1培养基中血清补充剂（合成血清替代物；SSS；欧文科学公司）对胚胎发育和着床的影响。
方法
根据取卵当天计划使用的培养基，将患者随机分为体外受精培养基组（第1组，周期数n = 172）或补充10% SSS的P1培养基组（第2组，周期数n = 229）。另外555个体外受精后续周期（第3组）使用P1培养基中增加的SSS浓度（20%）进行。
结果与结论
在这一大系列体外受精周期中，我们在此证明，与补充较低SSS浓度的培养基和体外受精培养基相比，当胚胎在补充20% SSS的无葡萄糖/磷酸盐培养基P1中培养时，妊娠率和着床率显著更高。
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