使用过氧化氢多用途隔离器进行人类胚胎培养基的内部制备：一项独特且成功的瑞士随机前瞻性研究。
The use of an hydrogen peroxide multipurpose isolator for inhouse preparation of human embryo culture media: a unique successful Swiss randomized prospective study.
作者信息
Van den Bergh Marc J G, Siragusa Antonino, Dubied André, Vonbach Priska, Deshe Mary Fahy, Flügel Klara, Ruflin Sandra, Stutz Jacqueline, Teufelberger Kirsten, Kratzer Anette, Urech Cornelia, Hohl Michael
机构信息
Fertility Laboratory, Frauenklinik, Kantonsspital Baden, Switzerland.
出版信息
J Assist Reprod Genet. 2004 Nov;21(11):381-6. doi: 10.1007/s10815-004-7525-8.
PURPOSE
To develop inhouse made (IHM) embryo culture medium with a Multipurpose Isolator and compare the embryo development in a prospective randomized study with commercial media.
METHODS
Fertilization by intracytoplasmic single sperm injection (ICSI) of Metaphase II oocytes obtained after 96 controlled ovarian hyperstimulation cycles in patients not older than 37 years. Transfer of zygotes to IHM or commercial Cook Sydney IVF Cleavage medium (SIC) immediately after pronucleus observation. Evaluation of embryo cleavage and score, pregnancy, and implantation rate.
RESULTS
From 100 zygotes cultured in SIC, 61% were at the 4 cell stage 45 h after ICSI compared to 77% (78/101) in the IHM, P < 0.05. The mean embryo score with IHM was 3.9 +/- 0.9 compared to 3.5 +/- 1.2 with SIC, P < 0.05. The clinical pregnancy rate per transfer was 38.9% (37/95), the implantation rate was 23% (46/200), and no differences were observed between the groups.
目的
使用多功能隔离器研发自制胚胎培养基（IHM），并在一项前瞻性随机研究中与商业培养基比较胚胎发育情况。
方法
对年龄不超过37岁的患者，在96个控制性卵巢过度刺激周期后获取的中期II卵母细胞进行卵胞浆内单精子注射（ICSI）受精。在原核观察后立即将受精卵转移至IHM或商业Cook悉尼IVF卵裂培养基（SIC）中。评估胚胎卵裂、评分、妊娠和着床率。
结果
在SIC中培养的100个受精卵中，ICSI后45小时有61%处于4细胞期，而在IHM中为77%（78/101），P<0.05。IHM组的平均胚胎评分为3.9±0.9，而SIC组为3.5±1.2，P<0.05。每次移植的临床妊娠率为38.9%（37/95），着床率为23%（46/200），两组之间未观察到差异。
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