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利马豆和刀豆种子的面粉及蛋白质分离物的功能特性

Functional properties of flours and protein isolates from Phaseolus lunatus and Canavalia ensiformis seeds.

作者信息

Chel-Guerrero L, Pérez-Flores V, Betancur-Ancona D, Dávila-Ortiz G

机构信息

Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av, Juárez No. 421 Cd. Industrial, 97288 Mérida, Yucatán, México.

出版信息

J Agric Food Chem. 2002 Jan 30;50(3):584-91. doi: 10.1021/jf010778j.


DOI:10.1021/jf010778j
PMID:11804533
Abstract

The functional properties of flours and protein isolates from the legumes Phaseolus lunatus and Canavalia ensiformis were evaluated to determine their potential practical applications. The respective protein isolates were obtained from the flours by using isoelectric precipitation, with a protein content of 71.13% for the P. lunatus isolate (PPI) and 73.75% for the C. ensiformis isolate (CPI). Nitrogen solubility was good in both acid and alkaline pHs for isolates and Canavalia flour (CF), with values as high as 80%, but not for the Phaseolus flour (PF). The flours and protein isolates had good water-holding capacities, with values between 2.65 and 3.80 g/g sample. Oil-holding capacity was highest in PPI (4.59 g/g sample) and CF (3.15 g/g sample). Under alkaline pH, the PPI foaming capacity (147%) was higher than those for CPI and CF, though the flours produced greater foam. Emulsifying activities for the PF, CF, PPI, and CPI were similar (46.78-53.84%) for pH range 6-10. Emulsion stability (ES) was superior in the CF and the CPI, where values reached 100% at pH 7 and 8. Apparent viscosity was pH-dependent.

摘要

对菜豆和刀豆这两种豆类的面粉及蛋白质分离物的功能特性进行了评估,以确定它们潜在的实际应用。通过等电沉淀从面粉中获得各自的蛋白质分离物,菜豆分离物(PPI)的蛋白质含量为71.13%,刀豆分离物(CPI)的蛋白质含量为73.75%。对于分离物和刀豆面粉(CF),在酸性和碱性pH条件下氮溶解度都很好,高达80%,但菜豆面粉(PF)并非如此。面粉和蛋白质分离物具有良好的持水能力,数值在2.65至3.80克/克样品之间。持油能力在PPI中最高(4.59克/克样品),在CF中为(3.15克/克样品)。在碱性pH条件下,PPI的起泡能力(147%)高于CPI和CF,不过面粉产生的泡沫更多。在pH值6至10范围内,PF、CF、PPI和CPI的乳化活性相似(46.78 - 53.84%)。乳液稳定性(ES)在CF和CPI中更优,在pH 7和8时数值达到100%。表观粘度取决于pH值。

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