以2-羟基-1-萘甲醛水杨酰腙（HNSH）为辣根过氧化物酶（HRP）的底物，用荧光分光光度法测定过氧化氢。
Spectrofluorimetric determination of hydrogen peroxide with 2-hydroxy-1-naphthaldehyde salicyloylhydrazone (HNSH) as the substrate for horseradish peroxidase (HRP).
作者信息
Tang Bo, Wang Yan, Sun Ying, Shen Han Xi
机构信息
Department of Chemistry, Shandong Normal University, Jinan, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2002 Jan 1;58(1):141-8. doi: 10.1016/s1386-1425(01)00514-5.
The oxidation reaction of HNSH with H2O2 under the catalysis of HRP was studied in detail. The possible reaction mechanism was discussed. Under optimum experimental conditions, the oxidized product of HNSH had excitation and emission maxima at 296 and 414 nm, respectively. A study to prove the existence of -O-O-H in polyethylene glycols was carried out. The proposed method was successfully applied to the determination of -O-O-H in polyethylene glycols.
详细研究了在辣根过氧化物酶催化下HNSH与H2O2的氧化反应。讨论了可能的反应机理。在最佳实验条件下，HNSH的氧化产物在296和414nm处分别有最大激发波长和发射波长。开展了一项关于证明聚乙二醇中存在-O-O-H的研究。所提出的方法成功应用于聚乙二醇中-O-O-H的测定。
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