Tang Bo, Zhang Li, Xu Ke-hua
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.
Talanta. 2006 Jan 15;68(3):876-82. doi: 10.1016/j.talanta.2005.06.053. Epub 2005 Aug 8.
A new kind of near-infrared fluorescence agent, tricarbochlorocyanine dye (Cy.7.Cl), had been synthesized in house and used for near-infrared spectrofluorimetric determination of hydrogen peroxide (H(2)O(2)) by flow injection analysis (FIA) for the first time. The oxidation reaction of Cy.7.Cl with H(2)O(2) occurred under the catalysis of horseradish peroxidase (HRP) and it was studied in detail. The possible reaction mechanism was discussed. Under optimal experimental conditions, fluorescence from Cy.7.Cl displayed excitation and emission maxima (ex/em) at 780 and 800 nm, respectively. The two linear working ranges were 1.86 x 10(-7) to 4.11 x 10(-7)mol L(-1) and 4.11 x 10(-7) to 7.19 x 10(-6)mol L(-1), respectively. The detection limit was 5.58 x 10(-8)mol L(-1) of H(2)O(2). The effect of interferences was studied. The proposed method was successfully applied to the determination of hydrogen peroxide in rainwater, serum and plant samples.
一种新型近红外荧光剂——三碳氯菁染料（Cy.7.Cl）已在实验室合成，并首次用于流动注射分析（FIA）近红外荧光光谱法测定过氧化氢（H₂O₂）。详细研究了Cy.7.Cl与H₂O₂在辣根过氧化物酶（HRP）催化下的氧化反应，并探讨了可能的反应机理。在最佳实验条件下，Cy.7.Cl的荧光激发和发射最大值（ex/em）分别为780和800 nm。两个线性工作范围分别为1.86×10⁻⁷至4.11×10⁻⁷mol L⁻¹和4.11×10⁻⁷至7.19×10⁻⁶mol L⁻¹。H₂O₂的检测限为5.58×10⁻⁸mol L⁻¹。研究了干扰的影响。该方法成功应用于雨水、血清和植物样品中过氧化氢的测定。
