Hsu Sheau Yu, Nakabayashi Koji, Nishi Shinya, Kumagai Jin, Kudo Masataka, Sherwood O David, Hsueh Aaron J W
Division of Reproductive Biology, Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Science. 2002 Jan 25;295(5555):671-4. doi: 10.1126/science.1065654.
Relaxin is a hormone important for the growth and remodeling of reproductive and other tissues during pregnancy. Although binding sites for relaxin are widely distributed, the nature of its receptor has been elusive. Here, we demonstrate that two orphan heterotrimeric guanine nucleotide binding protein (G protein)-coupled receptors, LGR7 and LGR8, are capable of mediating the action of relaxin through an adenosine 3',5'-monophosphate (cAMP)-dependent pathway distinct from that of the structurally related insulin and insulin-like growth factor family ligand. Treatment of antepartum mice with the soluble ligand-binding region of LGR7 caused parturition delay. The wide and divergent distribution of the two relaxin receptors implicates their roles in reproductive, brain, renal, cardiovascular, and other functions.
松弛素是一种在孕期对生殖及其他组织的生长和重塑很重要的激素。尽管松弛素的结合位点广泛分布，但其受体的性质一直难以捉摸。在此，我们证明了两个孤儿异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）偶联受体，LGR7和LGR8，能够通过一条与结构相关的胰岛素和胰岛素样生长因子家族配体不同的3',5'-环磷酸腺苷（cAMP）依赖性途径介导松弛素的作用。用LGR7的可溶性配体结合区域处理产前小鼠会导致分娩延迟。两种松弛素受体广泛且不同的分布表明它们在生殖、大脑、肾脏、心血管及其他功能中发挥作用。
