进展期胃癌的DNA倍体模式；它是一种临床可应用的预后指标吗？

DNA ploidy patterns in advanced gastric carcinoma; is it a clinically applicable prognosticator?

作者信息

Lee J H, Noh S H, Lee K Y, Choi S H, Min J S

机构信息

Department of Surgery, Yonsei University College of Medicine, 134, Shinchon-dong, Seodaemun-ku, 120-752, Seoul, Korea.

出版信息

Hepatogastroenterology. 2001 Nov-Dec;48(42):1793-6.

PMID:11813626

Abstract

BACKGROUND/AIMS: The purpose of this study was to evaluate the prognostic significance of ploidy patterns as determined by flow cytometry in terms of clinical usefulness.

METHODOLOGY

270 patients with a diagnosis of advanced gastric carcinoma were studied with fresh specimens obtained from multiple site avoiding nonviable or nonneoplastic tissues by DNA flow cytometry. DNA ploidy and clinicopathologic factors were compared and survival was analyzed.

RESULTS

The mean age of the patients was 56.1 +/- 11.6 years (25-80 years). There were 195 males and 75 females. Aneuploidy was shown in 93 (35.9%) patients. Male sex and differentiated tumor were more frequent in aneuploidy than diploidy (P = 0.011, < 0.001, respectively). By univariate analysis, tumor location, size, extent of resection, curative resection, serosa invasion, lymph node involvement, and distant metastasis significantly affected survival but not aneuploidy. Significant independent prognostic factors by multivariate analysis were curative resection, serosa invasion and lymph node involvement (P = 0.0001, 0.0114, 0.0262, respectively).

CONCLUSIONS

DNA ploidy patterns of advanced gastric carcinoma are of limited clinical significance, therefore not a clinically applicable prognosticator.

摘要

背景/目的：本研究旨在从临床实用性角度评估通过流式细胞术确定的倍体模式的预后意义。

方法

对270例诊断为进展期胃癌的患者进行研究，使用从多个部位获取的新鲜标本，通过DNA流式细胞术避免使用无活力或非肿瘤性组织。比较DNA倍体和临床病理因素，并分析生存率。

结果

患者的平均年龄为56.1±11.6岁（25 - 80岁）。男性195例，女性75例。93例（35.9%）患者显示为非整倍体。非整倍体患者中男性和分化型肿瘤比二倍体患者更常见（分别为P = 0.011，< 0.001）。单因素分析显示，肿瘤位置、大小、切除范围、根治性切除、浆膜侵犯、淋巴结受累和远处转移显著影响生存率，但非整倍体不影响。多因素分析显示，显著的独立预后因素是根治性切除、浆膜侵犯和淋巴结受累（分别为P = 0.0001，0.0114，0.0262）。