Anavi-Goffer Sharon, McKay Neil G, Ashford Michael L J, Coutts Angela A
Department of Biomedical Science, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK.
Neurosci Lett. 2002 Feb 8;319(1):53-7. doi: 10.1016/s0304-3940(01)02480-6.
The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers.
肠道感觉神经系统由外在和内在成分组成。在大鼠回肠、豚鼠回肠和结肠的肌间神经丛-纵行肌制备物中，研究了1型香草酸受体（VR1）的细胞分布及其与内在感觉神经元的关系。VR1免疫反应性定位于细纤维上，并由神经节细胞表达。在豚鼠肌间神经丛中，一部分VR1免疫反应性细胞与钙结合蛋白（一种内在传入神经元的标志物）共定位。用大鼠VR1特异性引物进行逆转录-聚合酶链反应，在大鼠制备物中检测到VR1 mRNA，但在豚鼠制备物中未检测到。我们得出结论，VR1在纤维和肌间神经元上表达。在豚鼠中，VR1由内在传入神经元表达，但其mRNA在引物区域可能与大鼠序列不同。
