Hind Anderson, Migliori Massimo, Thacker Michelle, Staikopoulos Vasiliki, Nurgali Kulmira, Chiocchetti Roberto, Furness John B
Department of Anatomy & Cell Biology, University of Melbourne, Parkville, Victoria, Australia.
Cell Tissue Res. 2005 Aug;321(2):151-7. doi: 10.1007/s00441-005-1129-1. Epub 2005 May 24.
The plant lectin, IB4, binds to the surfaces of primary afferent neurons of the dorsal root and trigeminal ganglia and is documented to be selective for nociceptive neurons. Physiological data suggest that the intrinsic primary afferent neurons within the intestine are also nociceptors. In this study, we have compared IB4 binding to each of these neuron types in the guinea-pig. The only neurons in the intestine to be readily revealed by IB4 binding have Dogiel-type-II morphology; these neurons have been previously identified as intrinsic primary afferent neurons. Most of the neurons that are IB4-positive in the myenteric plexus are calbindin-immunoreactive, whereas those in the submucosal ganglia are immunoreactive for NeuN. The neurons that bind IB4 strongly have a similar appearance in enteric, dorsal root and trigeminal ganglia. Binding is to the cell surface, to the first part of axons and to cytoplasmic organelles. A low level of binding was found in the extracellular matrix. A few other neurons in all ganglia exhibit faint staining with IB4. Strongly reactive neurons are absent from the gastric corpus. Thus, IB4 binding reveals primary afferent neurons with similar morphologies, patterns of binding and physiological roles in enteric, dorsal root and trigeminal ganglia.
植物凝集素IB4可与背根神经节和三叉神经节的初级传入神经元表面结合，并且据记载，它对伤害性神经元具有选择性。生理学数据表明，肠道内的固有初级传入神经元也是伤害感受器。在本研究中，我们比较了豚鼠中IB4与上述每种神经元类型的结合情况。肠道中仅有的可通过IB4结合而轻易显示的神经元具有多极II型形态；这些神经元先前已被确定为固有初级传入神经元。肌间神经丛中大多数IB4阳性神经元对钙结合蛋白呈免疫反应，而黏膜下神经节中的神经元对神经元核抗原呈免疫反应。在肠神经节、背根神经节和三叉神经节中，强烈结合IB4的神经元外观相似。结合发生在细胞表面、轴突的起始部分和细胞质细胞器上。在细胞外基质中发现了低水平的结合。所有神经节中的其他一些神经元对IB4呈微弱染色。胃体中不存在强反应性神经元。因此，IB4结合揭示了在肠神经节、背根神经节和三叉神经节中具有相似形态、结合模式和生理作用的初级传入神经元。
