Vaze A Y, Thakur A N, Sheth A R
J Reprod Fertil Suppl. 1979(26):135-46.
Using a homogeneous inhibin preparation from human seminal plasma with a molecular weight of about 19 000, a sensitive and specific radioimmunoassay (RIA) for inhibin has been developed. None of the purified hormones tested, such as LH, FSH and prolactin from different species, showed any cross-reaction in this RIA. Steroid hormones such as testosterone, dihydrotestosterone, oestradiol-17 beta and progesterone did not interfere with the assay. The antiserum had an affinity constant (Ka) of 2.379 X 10(9). The assay sensitivity was 10-15 ng per tube and the intra- and inter-assay coefficients of variation were 5-7% (n = 6) and 15% (n = 10) respectively. The recovery for inhibin added to the serum of a castrated man was 95-110%. Using this RIA, inhibin levels in various biological fluids and tissues were measured. Normo-spermic semen contained significantly higher levels of inhibin than did oligospermic semen. Human prostate contained a substantial quantity of inhibin. Monkey semen, rat serum, and bovine, ovine and porcine follicular fluids cross-reacted in the RIA, while ram testicular inhibin and bull semen did not do so. In developing (9-28 days of age) male rats, circulating inhibin levels showed an inverse relationship with serum FSH levels. In female rats of this age endogenous inhibin concentrations changed in parallel with those of serum FSH.
利用一种来自人精浆、分子量约为19000的纯一抑制素制剂，已开发出一种灵敏且特异的抑制素放射免疫分析法（RIA）。所检测的各种纯化激素，如来自不同物种的促黄体生成素（LH）、促卵泡生成素（FSH）和催乳素，在该放射免疫分析法中均未显示出任何交叉反应。睾酮、双氢睾酮、雌二醇-17β和孕酮等甾体激素不干扰该测定。抗血清的亲和常数（Ka）为2.379×10⁹。该测定的灵敏度为每管10 - 15纳克，批内和批间变异系数分别为5 - 7%（n = 6）和15%（n = 10）。添加到去势男性血清中的抑制素回收率为95 - 110%。利用这种放射免疫分析法，测定了各种生物体液和组织中的抑制素水平。正常精子精液中的抑制素水平显著高于少精子精液。人前列腺含有大量抑制素。猴精液、大鼠血清以及牛、羊和猪的卵泡液在该放射免疫分析法中有交叉反应，而公羊睾丸抑制素和公牛精液则无此反应。在发育中的（9 - 28日龄）雄性大鼠中，循环抑制素水平与血清促卵泡生成素水平呈负相关。在这个年龄段的雌性大鼠中，内源性抑制素浓度与血清促卵泡生成素浓度平行变化。
