Maquelin K, Choo-Smith L P, Endtz H P, Bruining H A, Puppels G J
Laboratory for Intensive Care Research and Optical Spectroscopy, Department of General Surgery 10M. Erasmus University Rotterdam and University Hospital Rotterdam, "Dijkzigt," Rotterdam, The Netherlands.
J Clin Microbiol. 2002 Feb;40(2):594-600. doi: 10.1128/JCM.40.2.594-600.2002.
Candida species are important nosocomial pathogens associated with high mortality rates. Rapid detection and identification of Candida species can guide a clinician at an early stage to prescribe antifungal drugs or to adjust empirical therapy when resistant species are isolated. Confocal Raman microspectroscopy is highly suitable for the rapid identification of Candida species, since Raman spectra can be directly obtained from microcolonies on a solid culture medium after only 6 h of culturing. In this study, we have used a set of 42 Candida strains comprising five species that are frequently encountered in clinical microbiology to test the feasibility of the technique for the rapid identification of Candida species. The procedure was started either from a culture on Sabouraud medium or from a positive vial of an automated blood culture system. Prior to Raman measurements, strains were subcultured on Sabouraud medium for 6 h to form microcolonies. Using multivariate statistical analyses, a high prediction accuracy (97 to 100%) was obtained with the Raman method. Identification with Raman microspectroscopy may therefore be significantly faster than identification with commercial identification systems that allow various species to be identified and that often require 24 to 48 h before a reliable identification is obtained. We conclude that confocal Raman microspectroscopy offers a rapid, accurate, and easy-to-use alternative for the identification of clinically relevant Candida species.
念珠菌属是与高死亡率相关的重要医院感染病原体。快速检测和鉴定念珠菌属可在早期指导临床医生开具抗真菌药物，或在分离出耐药菌株时调整经验性治疗方案。共聚焦拉曼光谱非常适合快速鉴定念珠菌属，因为仅培养6小时后即可直接从固体培养基上的微菌落获得拉曼光谱。在本研究中，我们使用了一组42株念珠菌菌株，包括临床微生物学中常见的五个菌种，以测试该技术快速鉴定念珠菌属的可行性。该程序可以从沙保弱培养基上的培养物开始，也可以从自动血培养系统的阳性瓶开始。在进行拉曼测量之前，将菌株在沙保弱培养基上继代培养6小时以形成微菌落。使用多变量统计分析，拉曼方法获得了较高的预测准确率（97%至100%）。因此，与商业鉴定系统相比，拉曼光谱鉴定可能要快得多，商业鉴定系统可以鉴定多种菌种，通常需要24至48小时才能获得可靠的鉴定结果。我们得出结论，共聚焦拉曼光谱为鉴定临床相关念珠菌属提供了一种快速、准确且易于使用的替代方法。
