Maquelin K, Kirschner C, Choo-Smith L-P, van den Braak N, Endtz H Ph, Naumann D, Puppels G J
Laboratory for Intensive Care Research and Optical Spectroscopy, Department of General Surgery 10M, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands.
J Microbiol Methods. 2002 Nov;51(3):255-71. doi: 10.1016/s0167-7012(02)00127-6.
In the recent years, vibrational spectroscopies (infrared and Raman spectroscopy) have been developed for all sorts of analyses in microbiology. Important features of these methods are the relative ease with which measurements can be performed. Furthermore, in order to obtain infrared or Raman spectra, there is only a limited amount of sample handling involved without the need for expensive chemicals, labels or dyes. Here, we review the potential application of vibrational spectroscopies for the use in medical microbiology. After describing some of the basics of the techniques, considerations on reproducibility and standardisation are presented. Finally, the use of infrared and Raman spectroscopy for the (rapid) identification of medically relevant microorganisms is discussed. It can be concluded that vibrational spectroscopies show high potential as novel methods in medical microbiology.
近年来，振动光谱法（红外光谱和拉曼光谱）已被开发用于微生物学的各种分析。这些方法的重要特点是测量相对容易进行。此外，为了获得红外或拉曼光谱，只需进行有限的样品处理，无需使用昂贵的化学品、标记物或染料。在此，我们综述了振动光谱法在医学微生物学中的潜在应用。在描述了这些技术的一些基础知识后，提出了关于可重复性和标准化的考虑因素。最后，讨论了使用红外和拉曼光谱法（快速）鉴定医学相关微生物的情况。可以得出结论，振动光谱法作为医学微生物学中的新方法具有很高的潜力。
