Graf B, Adam T, Zill E, Göbel U B
Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, 10117 Berlin, Germany.
J Clin Microbiol. 2000 May;38(5):1782-5. doi: 10.1128/JCM.38.5.1782-1785.2000.
The new VITEK 2 system is a fully automated system dedicated to the identification and susceptibility testing of microorganisms. In conjunction with the VITEK ID-YST card the VITEK 2 system allows the identification of clinically important yeasts and yeast-like organisms in 15 h due to a sensitive fluorescence-based technology. The ID-YST card consists of 47 biochemical reactions. The database comprises 51 taxa, including newly described species. In this study we evaluated the reliability of the VITEK ID-YST card for the identification of yeasts and yeast-like organisms encountered in a clinical microbiology laboratory. A total of 241 strains representing 21 species were studied. The strains were isolated from clinical samples within a period of 60 days prior to the identification. The tests were performed using 24-h to 55-h subcultures on Sabouraud-gentamicin-chloramphenicol agar. Each strain was tested in parallel using the ID 32C strip as a comparison method combined with microscopic morphology and an agglutination test for C. krusei. Overall, 222 strains (92.1%) were unequivocally identified including 11 isolates (4.6%) identified with low discrimination resolved by simple additional tests. Ten strains (4. 1%) for which results were given with low discrimination could not be unequivocally identified with supplemental tests, 4 strains (1. 7%) were misidentified and 5 strains (2.1%) could not be identified. In conclusion, we found that the VITEK 2 system is a rapid and accurate method for the identification of medically important yeasts and yeast-like organisms.
新型VITEK 2系统是一个专门用于微生物鉴定和药敏试验的全自动系统。结合VITEK ID-YST卡，VITEK 2系统凭借基于荧光的灵敏技术，可在15小时内鉴定出具有临床意义的酵母和类酵母微生物。ID-YST卡包含47种生化反应。数据库涵盖51个分类单元，包括新描述的物种。在本研究中，我们评估了VITEK ID-YST卡在临床微生物实验室中鉴定酵母和类酵母微生物的可靠性。共研究了代表21个物种的241株菌株。这些菌株是在鉴定前60天内从临床样本中分离出来的。试验在含有沙氏-庆大霉素-氯霉素的琼脂上进行24小时至55小时的传代培养后开展。每种菌株同时使用ID 32C试条进行测试，作为一种比较方法，并结合显微镜形态学以及针对克鲁斯假丝酵母菌的凝集试验。总体而言，222株菌株（92.1%）得到明确鉴定，其中11株分离株（4.6%）鉴定结果鉴别力低，通过简单的附加试验得以解决。10株菌株（4.1%）鉴定结果鉴别力低，通过补充试验仍无法明确鉴定，4株菌株（1.7%）被错误鉴定，5株菌株（2.1%）无法鉴定。总之，我们发现VITEK 2系统是鉴定医学上重要的酵母和类酵母微生物的一种快速且准确的方法。
