Chen Chih-Yu, Chou Hong-Nong, Chen Yih-Min, Lee Tzong-Huei
Science and Technology Information Center, National Science Council, Taipei, Taiwan, ROC.
J Nat Toxins. 2002 Feb;11(1):63-8.
High performance liquid chromatography (HPLC) using fluorescent detection following post-column alkaline degradation and a sample preparation procedure for the analysis were established to detect tetrodotoxins (TTXs) in seafood. In south Taiwan Prefectures, each specimen of shellfishes and gobies, collected from Chiayi, Tainan, and Pingtung from January 1997 to May 1998, was analyzed by HPLC to detect the presence and quantity of TTXs. Overall results showed that only 5 specimens out of 557 specimens contained TTXs; the toxic species were gastropod Natica lineata and Nassarius livescens. The highest TTX content is 10.0 microg/g in N. livescens. Gobies and other species of shellfishes were nontoxic. Although the rate of toxic specimens in all samples was low and showed no seasonal trends, the TTX contents of toxic specimens were higher than safety criteria value.
建立了柱后碱性降解后采用荧光检测的高效液相色谱法（HPLC）以及用于分析的样品制备程序，以检测海鲜中的河豚毒素（TTXs）。在台湾南部各县，从1997年1月至1998年5月从嘉义、台南和屏东采集的每一份贝类和虾虎鱼标本，都通过HPLC进行分析，以检测TTXs的存在和含量。总体结果显示，在557份标本中只有5份含有TTXs；有毒物种是腹足纲的纵带织纹螺和彩虹织纹螺。彩虹织纹螺中的TTX最高含量为10.0微克/克。虾虎鱼和其他贝类物种无毒。尽管所有样本中有毒标本的比例较低且无季节性趋势，但有毒标本的TTX含量高于安全标准值。
